ArticleFrontiers in cellular neuroscience2013
Synaptic vesicle capture by CaV2.2 calcium channels.
Article in Frontiers in cellular neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Distinct active zone protein machineries mediate CaNature neuroscience · 2024Article
- Microelectrode Arrays Measure Blocking of Voltage-Gated Calcium Ion Channels on Supported Lipid Bilayers Derived from Primary Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Molecular definition of distinct active zone protein machineries for CabioRxiv : the preprint server for biology · 2023Article
- Effect of semaglutide and empagliflozin on cognitive function and hippocampal phosphoproteomic in obese mice.Frontiers in pharmacology · 2023Article
- Presynaptic voltage-gated calcium channels in the auditory brainstem.Molecular and cellular neurosciences · 2021Review
- Neurotransmitter Release Site Replenishment and Presynaptic Plasticity.International journal of molecular sciences · 2020Review
- Interactions of Rabconnectin-3 with Cav2 calcium channels.Molecular brain · 2019Article
- Molecular Characterization of an SV Capture Site in the Mid-Region of the Presynaptic CaV2.1 Calcium Channel C-Terminal.Frontiers in cellular neuroscience · 2018Article
- A novel region in the CaeLife · 2017Article
- The Calcium Channel C-Terminal and Synaptic Vesicle Tethering: Analysis by Immuno-Nanogold Localization.Frontiers in cellular neuroscience · 2017Article
- Variable priming of a docked synaptic vesicle.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Characterization of a Synaptic Vesicle Binding Motif on the Distal CaV2.2 Channel C-terminal.Frontiers in cellular neuroscience · 2016Article
- The Physiology, Pathology, and Pharmacology of Voltage-Gated Calcium Channels and Their Future Therapeutic Potential.Pharmacological reviews · 2015Review
- The structure and function of 'active zone material' at synapses.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Single calcium channel domain gating of synaptic vesicle fusion at fast synapses; analysis by graphic modeling.Channels (Austin, Tex.) · 2015Article
- Synaptic vesicle tethering and the CaV2.2 distal C-terminal.Frontiers in cellular neuroscience · 2014Article
- Cryoloading: introducing large molecules into live synaptosomes.Frontiers in cellular neuroscience · 2014Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fusion of synaptic vesicles (SVs) at the presynaptic transmitter release face is gated by Ca(2) (+) influx from nearby voltage-gated calcium channels (CaVs). Functional studies favor a direct molecular "tethering" attachment and recent studies have proposed a direct link to the channel C-terminal. To test for direct CaV-SV attachment we developed an in vitro assay, termed SV pull-down (SV-PD), to test for capture of purified, intact SVs. Antibody-immobilized presynaptic or expressed CaV2.2 channels but not plain beads, IgG or pre-blocked antibody successfully captured SVs, as assessed byWestern blot for a variety of protein markers. SV-PD was also observed with terminal fusion proteins of the distal half of the C-terminal, supporting involvement of this CaV region in tethering. Thus our results support a model in which the SV tethers directly to the CaV. Since the tip of the C-terminal could extend as far as 200 nm into the cytoplasm, we hypothesize that this link may serve as the initial SV capture mechanism by the release site. Further studies will be necessary to evaluate the molecular basis of C-terminal tethering and whether the SV binds to the channel by additional, shorter-range attachments.
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