ArticleThe Journal of biological chemistry2013
Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
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- Function of left ventricle mitochondria in highland deer mice and lowland mice.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Lactate shuttling as an allostatic means of thermoregulation in the brain.Frontiers in neuroscience · 2023Article
- From rags to riches: Lactate ascension as a pivotal metabolite in neuroenergetics.Frontiers in neuroscience · 2023Article
- Comparative Transcriptomic Analysis of mRNAs, miRNAs and lncRNAs in theBiomolecules · 2022Article
- Assessment of catabolic state in infants with the use of urinary titin N-fragment.Pediatric research · 2022Article
- Mitochondrial Transport in Glycolysis and Gluconeogenesis: Achievements and Perspectives.International journal of molecular sciences · 2021Review
- Changes in energetic metabolism and lysosomal destruction in the skeletal muscle and cardiac tissues of pigeons (Columba livia f. urbana) from urban areas of the northern Pomeranian region (Poland).Ecotoxicology (London, England) · 2021Article
- Mitochondrial lactate metabolism: history and implications for exercise and disease.The Journal of physiology · 2021Review
- The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice.Frontiers in physiology · 2021Article
- Lactate dehydrogenase supports lactate oxidation in mitochondria isolated from different mouse tissues.Redox biology · 2020Article
- Metabolic engineering ofSynthetic and systems biotechnology · 2019Article
- Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts.GeroScience · 2018Article
- Lactate metabolism: historical context, prior misinterpretations, and current understanding.European journal of applied physiology · 2018Review
- Glycolysis Paradigm Shift Dictates a Reevaluation of Glucose and Oxygen Metabolic Rates of Activated Neural Tissue.Frontiers in neuroscience · 2018Article
- Effects of naringin on physical fatigue and serum MMP-9 concentration in female rats.Pharmaceutical biology · 2017Article
- Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.The Journal of biological chemistry · 2016Article
- Lactate metabolism is associated with mammalian mitochondria.Nature chemical biology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular lactate shuttle hypothesis posits that lactate generated in the cytosol is oxidized by mitochondrial lactate dehydrogenase (LDH) of the same cell. To examine whether skeletal muscle mitochondria oxidize lactate, mitochondrial respiratory oxygen flux (JO2) was measured during the sequential addition of various substrates and cofactors onto permeabilized rat gastrocnemius muscle fibers, as well as isolated mitochondrial subpopulations. Addition of lactate did not alter JO2. However, subsequent addition of NAD(+) significantly increased JO2, and was abolished by the inhibitor of mitochondrial pyruvate transport, α-cyano-4-hydroxycinnamate. In experiments with isolated subsarcolemmal and intermyofibrillar mitochondrial subpopulations, only subsarcolemmal exhibited NAD(+)-dependent lactate oxidation. To further investigate the details of the physical association of LDH with mitochondria in muscle, immunofluorescence/confocal microscopy and immunoblotting approaches were used. LDH clearly colocalized with mitochondria in intact, as well as permeabilized fibers. LDH is likely localized inside the outer mitochondrial membrane, but not in the mitochondrial matrix. Collectively, these results suggest that extra-matrix LDH is strategically positioned within skeletal muscle fibers to functionally interact with mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.