ArticleApplied and environmental microbiology2013
Comparative proteomic analysis of Streptomyces lividans Wild-Type and ppk mutant strains reveals the importance of storage lipids for antibiotic biosynthesis.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 31 citations in OpenAlex.
- Autonomous Defense Based on Biogenic Nanoparticle Formation in Daunomycin-ProducingMicroorganisms · 2025Article
- The DeoR-like pleiotropic regulator SCO1897 controls specialised metabolism, sporulation, spore germination, and phosphorus accumulation in Streptomyces coelicolor.Communications biology · 2024Article
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- The Phosin PptA Plays a Negative Role in the Regulation of Antibiotic Production inAntibiotics (Basel, Switzerland) · 2021Article
- System-level understanding of gene expression and regulation for engineering secondary metabolite production in Streptomyces.Journal of industrial microbiology & biotechnology · 2020Review
- Negative Correlation between Lipid Content and Antibiotic Activity inAntibiotics (Basel, Switzerland) · 2020Article
- Expression of genes of the Pho regulon is altered in Streptomyces coelicolor.Scientific reports · 2020Article
- Unlocking a new target for streptomycetes strain improvement.Synthetic and systems biotechnology · 2020Article
- The Inhibition of Antibiotic Production inFrontiers in microbiology · 2020Article
- Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold.FEBS letters · 2019Article
- The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.Microbial cell factories · 2018Article
- Regulation of specialised metabolites in Actinobacteria - expanding the paradigms.Environmental microbiology reports · 2018Review
- Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145.Scientific reports · 2017Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces lividans TK24 is a strain that naturally produces antibiotics at low levels, but dramatic overproduction of antibiotics occurs upon interruption of the ppk gene. However, the role of the Ppk enzyme in relation to the regulation of antibiotic biosynthesis remains poorly understood. In order to gain a better understanding of the phenotype of the ppk mutant, the proteomes of the wild-type (wt) and ppk mutant strains, grown for 96 h on R2YE medium limited in phosphate, were analyzed. Intracellular proteins were separated on two-dimensional (2D) gels, spots were quantified, and those showing a 3-fold variation or more were identified by mass spectrometry. The expression of 12 proteins increased and that of 29 decreased in the ppk mutant strain. Our results suggested that storage lipid degradation rather than hexose catabolism was taking place in the mutant. In order to validate this hypothesis, the triacylglycerol contents of the wt and ppk mutant strains of S. lividans as well as that of Streptomyces coelicolor M145, a strain that produces antibiotics at high levels and is closely related to S. lividans, were assessed using electron microscopy and thin-layer chromatography. These studies highlighted the large difference in triacylglycerol contents of the three strains and confirmed the hypothetical link between storage lipid metabolism and antibiotic biosynthesis in Streptomyces.
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