ArticleApplied and environmental microbiology2013
Detoxification of mercury by methanobactin from Methylosinus trichosporium OB3b.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Methanobactins: Structures, Biosynthesis, and Microbial Diversity.Annual review of microbiology · 2024Review
- Crystal structure of MbnF: an NADPH-dependent flavin monooxygenase from Methylocystis strain SB2.Acta crystallographica. Section F, Structural biology communications · 2023Article
- Article
- Article
- Two TonB-Dependent Transporters in Methylosinus trichosporium OB3b Are Responsible for Uptake of Different Forms of Methanobactin and Are Involved in the Canonical "Copper Switch".Applied and environmental microbiology · 2022Article
- Antimicrobial Activity of Metals and Metalloids.Annual review of microbiology · 2021Review
- Metal(loid) speciation and transformation by aerobic methanotrophs.Microbiome · 2021Review
- Detoxification, Active Uptake, and Intracellular Accumulation of Chromium Species by a Methane-Oxidizing Bacterium.Applied and environmental microbiology · 2021Article
- Genomic and Physiological Properties of a Facultative Methane-Oxidizing Bacterial Strain ofMicroorganisms · 2020Article
- Synergistic Effects of a Chalkophore, Methanobactin, on Microbial Methylation of Mercury.Applied and environmental microbiology · 2020Article
- Enhanced Adsorptive Bioremediation of Heavy Metals (CdMicroorganisms · 2020Article
- Microbial generation of elemental mercury from dissolved methylmercury in seawater.Limnology and oceanography · 2019Article
- Chalkophores.Annual review of biochemistry · 2018Review
- Methanobactins: Maintaining copper homeostasis in methanotrophs and beyond.The Journal of biological chemistry · 2018Review
- Metals and Methanotrophy.Applied and environmental microbiology · 2018Review
- Metagenomic Evidence for aFrontiers in microbiology · 2018Article
- Methylmercury uptake and degradation by methanotrophs.Science advances · 2017Article
- Methanobactins: from genome to function.Metallomics : integrated biometal science · 2017Review
- Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b.Metallomics : integrated biometal science · 2016Article
- Methanobactin and the Link between Copper and Bacterial Methane Oxidation.Microbiology and molecular biology reviews : MMBR · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many methanotrophs have been shown to synthesize methanobactin, a novel biogenic copper-chelating agent or chalkophore. Methanobactin binds copper via two heterocyclic rings with associated enethiol groups. The structure of methanobactin suggests that it can bind other metals, including mercury. Here we report that methanobactin from Methylosinus trichosporium OB3b does indeed bind mercury when added as HgCl2 and, in doing so, reduced toxicity associated with Hg(II) for both Alphaproteobacteria methanotrophs, including M. trichosporium OB3b, M. trichosporium OB3b ΔmbnA (a mutant defective in methanobactin production), and Methylocystis sp. strain SB2, and a Gammaproteobacteria methanotroph, Methylomicrobium album BG8. Mercury binding by methanobactin was evident in both the presence and absence of copper, despite the fact that methanobactin had a much higher affinity for copper due to the rapid and irreversible binding of mercury by methanobactin. The formation of a gray precipitate suggested that Hg(II), after being bound by methanobactin, was reduced to Hg(0) but was not volatilized. Rather, mercury remained associated with methanobactin and was also found associated with methanotrophic biomass. It thus appears that although the mercury-methanobactin complex was cell associated, mercury was not removed from methanobactin. The amount of biomass-associated mercury in the presence of methanobactin from M. trichosporium OB3b was greatest for M. trichosporium wild-type strain OB3b and the ΔmbnA mutant and least for M. album BG8, suggesting that methanotrophs may have selective methanobactin uptake systems that may be based on TonB-dependent transporters but that such uptake systems exhibit a degree of infidelity.
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