ArticleCancer cell international2013
Release and uptake of volatile organic compounds by human hepatocellular carcinoma cells (HepG2) in vitro.
Article in Cancer cell international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- Urinary and cellular volatile organic compounds as biomarkers for urological cancers: a systematic review of GC-MS-based volatolomics.Metabolomics : Official journal of the Metabolomic Society · 2026Pooled it
- Metabonomics fingerprint of volatile organic compounds in serum and urine of pregnant women with gestational diabetes mellitus.World journal of diabetes · 2022Trial
- Urinary Volatile Organic Compounds as Non-Invasive Biomarkers to Distinguish Hepatocellular Carcinoma from Cirrhosis: A Multicentre Diagnostic Accuracy Study.Sensors (Basel, Switzerland) · 2026Observational
- Volatilomic Signatures of Parental and Oxaliplatin-Resistant HCT116 Colon Cancer Cell Lines.International journal of molecular sciences · 2026Article
- From Industry to Dentistry: A Comprehensive Review of Zeolite as a Next-Generation Multifunctional Filler for Enhanced Mechanical Reinforcement and Antimicrobial Efficacy.Dentistry journal · 2025Review
- Time-resolved monitoring of yeast responses to lipopolysaccharide exposure by cell-released volatile organic compounds.Applied and environmental microbiology · 2025Article
- Stimulated C2C12 Myotube Headspace Volatile Organic Compound Analysis.Molecules (Basel, Switzerland) · 2024Article
- Unveiling Colorectal Cancer Biomarkers: Harnessing Biosensor Technology for Volatile Organic Compound Detection.Sensors (Basel, Switzerland) · 2024Review
- Volatile organic compounds for the detection of hepatocellular carcinoma-A scoping review.iLIVER · 2024Review
- GC/MS analysis of hypoxic volatile metabolic markers in the MDA-MB-231 breast cancer cell line.Frontiers in molecular biosciences · 2023Article
- Diagnosis by Volatile Organic Compounds in Exhaled Breath in Exhaled Breath from Patients with Gastric and Colorectal Cancers.International journal of molecular sciences · 2022Review
- Sampling and Analysis of Low-Molecular-Weight Volatile Metabolites in Cellular Headspace and Mouse Breath.Metabolites · 2022Article
- Volatilomic Signatures of AGS and SNU-1 Gastric Cancer Cell Lines.Molecules (Basel, Switzerland) · 2022Article
- Exploratory Study Using Urinary Volatile Organic Compounds for the Detection of Hepatocellular Carcinoma.Molecules (Basel, Switzerland) · 2021Article
- The Volatilomic Footprints of Human HGC-27 and CLS-145 Gastric Cancer Cell Lines.Frontiers in molecular biosciences · 2020Article
- Volatile scents of influenza A and S. pyogenes (co-)infected cells.Scientific reports · 2019Article
- Article
- Profiling Single Cancer Cells with Volatolomics Approach.iScience · 2019Article
- Digging deeper into volatile organic compounds associated with cancer.Biology methods & protocols · 2019Review
- VOC breath profile in spontaneously breathing awake swine during Influenza A infection.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundVolatile organic compounds (VOCs) emitted by human body offer a unique insight into biochemical processes ongoing in healthy and diseased human organisms. Unfortunately, in many cases their origin and metabolic fate have not been yet elucidated in sufficient depth, thus limiting their clinical application. The primary goal of this work was to identify and quantify volatile organic compounds being released or metabolized by HepG2 hepatocellular carcinoma cells.
methodsThe hepatocellular carcinoma cells were incubated in specially designed head-space 1-L glass bottles sealed for 24 hours prior to measurements. Identification and quantification of volatiles released and consumed by cells under study were performed by gas chromatography with mass spectrometric detection (GC-MS) coupled with head-space needle trap device extraction (HS-NTD) as the pre-concentration technique. Most of the compounds were identified both by spectral library match as well as retention time comparison based on standards.
resultsA total of nine compounds were found to be metabolised and further twelve released by the cells under study (Wilcoxon signed-rank test, p<0.05). The former group comprised 6 aldehydes (2-methyl 2-propenal, 2-methyl propanal, 2-ethylacrolein, 3-methyl butanal, n-hexanal and benzaldehyde), n-propyl propionate, n-butyl acetate, and isoprene. Amongst the released species there were five ketones (2-pentanone, 3-heptanone, 2-heptanone, 3-octanone, 2-nonanone), five volatile sulphur compounds (dimethyl sulfide, ethyl methyl sulfide, 3-methyl thiophene, 2-methyl-1-(methylthio)- propane and 2-methyl-5-(methylthio) furan), n-propyl acetate, and 2-heptene.
conclusionsThe emission and uptake of the aforementioned VOCs may reflect the activity of abundant liver enzymes and support the potential of VOC analysis for the assessment of enzymes function.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.