ArticleBiophysical journal2013
Statistical analysis of peptide-induced graded and all-or-none fluxes in giant vesicles.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Predicting peptide orientation in membrane interactions: a review on transmembrane and surface-bound states.European biophysics journal : EBJ · 2026Review
- A unified model of transient poration induced by antimicrobial peptides.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Story of Pore-Forming Proteins from Deadly Disease-Causing Agents to Modern Applications with Evolutionary Significance.Molecular biotechnology · 2024Review
- The Important Role of Membrane Fluidity on the Lytic Mechanism of the α-Pore-Forming Toxin Sticholysin I.Toxins · 2023Article
- Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration.Nature communications · 2022Article
- Standardizing characterization of membrane active peptides with microfluidics.Biomicrofluidics · 2021Article
- Attacins: A Promising Class of Insect Antimicrobial Peptides.Antibiotics (Basel, Switzerland) · 2021Review
- The Antibiotic Peptide Daptomycin Functions by Reorganizing the Membrane.The Journal of membrane biology · 2021Review
- Using fluorescence microscopy to shed light on the mechanisms of antimicrobial peptides.Future medicinal chemistry · 2019Review
- Mechanistic Landscape of Membrane-Permeabilizing Peptides.Chemical reviews · 2019Review
- The Mechanism of Membrane Permeabilization by Peptides: Still an Enigma.Australian journal of chemistry · 2019Article
- Daptomycin-Phosphatidylglycerol Domains in Lipid Membranes.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Charge Distribution Fine-Tunes the Translocation of α-Helical Amphipathic Peptides across Membranes.Biophysical journal · 2016Article
- Correlating antimicrobial activity and model membrane leakage induced by nylon-3 polymers and detergents.Soft matter · 2015Article
- Membrane-active peptides: binding, translocation, and flux in lipid vesicles.Biochimica et biophysica acta · 2014Review
- Implicit membrane treatment of buried charged groups: application to peptide translocation across lipid bilayers.Biochimica et biophysica acta · 2014Article
- Additive and synergistic membrane permeabilization by antimicrobial (lipo)peptides and detergents.Biophysical journal · 2014Article
- Translocation of cationic amphipathic peptides across the membranes of pure phospholipid giant vesicles.Journal of the American Chemical Society · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Antimicrobial, cytolytic, and cell-penetrating peptides induce pores or perturbations in phospholipid membranes that result in fluxes of dyes into or out of lipid vesicles. Here we examine the fluxes induced by four of these membrane-active peptides in giant unilamellar vesicles. The type of flux is determined from the modality of the distributions of vesicles as a function of their dye content using the statistical Hartigan dip test. Graded and all-or-none fluxes correspond to unimodal and bimodal distributions, respectively. To understand how these distributions arise, we perform Monte Carlo simulations of peptide-induced dye flux into vesicles using a very simple model. The modality of the distributions depends on the rate constants of pore opening and closing, and dye flux. If the rate constants of pore opening and closing are both much smaller than that of dye flux through the pore, all-or-none influx occurs. However, if one of them, especially the rate constant for pore opening, increases significantly relative to the flux rate constant, the process becomes graded. In the experiments, we find that the flux type is the same in giant and large vesicles, for all peptides except one. But this one exception indicates that the flux type cannot be used to unambiguously predict the mechanism of membrane permeabilization by the peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.