ArticleBiophysical journal2013
Individual vesicle fusion events mediated by lipid-anchored DNA.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 82 citations in OpenAlex.
- From Population Averaging to Single Event Resolution: Evolution of Sensing Platforms for Membrane Fusion.Sensors (Basel, Switzerland) · 2026Review
- Functionalizing Nucleic Acids: Synthesis and Purification Strategies for Bioconjugates as Biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Boosting Lipofection Efficiency Through Enhanced Membrane Fusion Mechanisms.International journal of molecular sciences · 2024Review
- Programmable RNA Loading of Extracellular Vesicles with Toehold-Release Purification.Journal of the American Chemical Society · 2024Article
- SARS-CoV-2 and Epstein-Barr Virus-like Particles Associate and Fuse with Extracellular Vesicles in Virus Neutralization Tests.Biomedicines · 2023Article
- Dynamic Fusion of Nucleic Acid Functionalized Nano-/Micro-Cell-Like Containments: From Basic Concepts to Applications.ACS nano · 2023Review
- Designer Nanodiscs to Probe and Reprogram Membrane Biology in Synapses.Journal of molecular biology · 2023Review
- Probing the Separation Distance between Biological Nanoparticles and Cell Membrane Mimics Using Neutron Reflectometry with Sub-Nanometer Accuracy.Journal of the American Chemical Society · 2022Article
- DNA-assisted selective electrofusion (DASE) ofNanoscale · 2022Article
- Single-particle combinatorial multiplexed liposome fusion mediated by DNA.Nature chemistry · 2022Article
- Vesicle-based artificial cells: materials, construction methods and applications.Materials horizons · 2022Review
- Modulating the Influenza A Virus-Target Membrane Fusion Interface With Synthetic DNA-Lipid Receptors.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Single-virus content-mixing assay reveals cholesterol-enhanced influenza membrane fusion efficiency.Biophysical journal · 2021Article
- Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles.Nature protocols · 2021Review
- Cations Regulate Membrane Attachment and Functionality of DNA Nanostructures.Journal of the American Chemical Society · 2021Article
- Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.Biophysical journal · 2020Article
- Interaction of SNARE Mimetic Peptides with Lipid bilayers: Effects of Secondary Structure, Bilayer Composition and Lipid Anchoring.Scientific reports · 2019Article
- Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugates as a surrogate for the membrane proteins involved in the biological fusion reaction. We previously showed that complementary DNA-lipids, inserted into small unilamellar vesicles, can mediate membrane fusion in bulk. Here, we use a model membrane architecture developed in our lab to directly observe single-vesicle fusion events using fluorescence microscopy. In this system, a planar tethered membrane patch serves as the target membrane for incoming vesicles. This allows us to quantify the kinetics and characteristics of individual fusion events from the perspective of the lipids or the DNA-lipids involved in the process. We find that the fusion pathways are heterogeneous, with an arrested hemi-fusion state predominating, and we quantitate the outcome and rate of fusion events to construct a mechanistic model of DNA-mediated vesicle fusion. The waiting times between docking and fusion are distributed exponentially, suggesting that fusion occurs in a single step. Our analysis indicates that when two lipid bilayers are brought into close proximity, fusion occurs spontaneously, with little or no dependence on the number of DNA hybrids formed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.