ArticleBiophysical journal2013
Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- EPR spectroscopy 80 years after its discovery.Science advances · 2026Review
- Field-domain rapid-scan EPR at 240GHz for studies of protein functional dynamics at room temperature.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- Rapid scan EPR: Automated digital resonator control for low-latency data acquisition.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2022Article
- Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles.International journal of molecular sciences · 2022Article
- Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.Pharmaceutics · 2021Review
- Nanoantioxidants: Pioneer Types, Advantages, Limitations, and Future Insights.Molecules (Basel, Switzerland) · 2021Review
- Rapid-scan electron paramagnetic resonance using an EPR-on-a-Chip sensor.Magnetic resonance (Gottingen, Germany) · 2021Article
- EPR Everywhere.Applied magnetic resonance · 2021Review
- Rapid-Scan Electron Paramagnetic Resonance of Highly Resolved Hyperfine Lines in Organic Radicals.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Article
- Rapid Scan EPR imaging as a Tool for Magnetic Field Mapping.Applied magnetic resonance · 2020Article
- Monitoring Protein-Protein Interactions in the Cyanobacterial Circadian Clock in Real Time via Electron Paramagnetic Resonance Spectroscopy.Biochemistry · 2020Article
- Full cycle rapid scan EPR deconvolution algorithm.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2017Article
- Using rapid-scan EPR to improve the detection limit of quantitative EPR by more than one order of magnitude.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2017Article
- Rapid-scan EPR imaging.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2017Review
- Spin Trapping: A Review for the Study of Obesity Related Oxidative Stress and NaJournal of clinical & cellular immunology · 2017Article
- Concept of Phase Cycling in Pulsed Magnetic Resonance Using Sinusoidal Magnetic Field Modulation.Zeitschrift fur physikalische Chemie (Frankfurt am Main, Germany) · 2017Article
- Detection of Reactive Oxygen and Nitrogen Species by Electron Paramagnetic Resonance (EPR) Technique.Molecules (Basel, Switzerland) · 2017Review
- Accelerated dynamic EPR imaging using fast acquisition and compressive recovery.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2016Article
- Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo.Journal of visualized experiments : JoVE · 2016Article
- Rapid scan electron paramagnetic resonance at 1.0 GHz of defect centers in γ-irradiated organic solids.Radiation measurements · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The short lifetime of superoxide and the low rates of formation expected in vivo make detection by standard continuous wave (CW) electron paramagnetic resonance (EPR) challenging. The new rapid-scan EPR method offers improved sensitivity for these types of samples. In rapid-scan EPR, the magnetic field is scanned through resonance in a time that is short relative to electron spin relaxation times, and data are processed to obtain the absorption spectrum. To validate the application of rapid-scan EPR to spin trapping, superoxide was generated by the reaction of xanthine oxidase and hypoxanthine with rates of 0.1-6.0 μM/min and trapped with 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO). Spin trapping with BMPO to form the BMPO-OOH adduct converts the very short-lived superoxide radical into a more stable spin adduct. There is good agreement between the hyperfine splitting parameters obtained for BMPO-OOH by CW and rapid-scan EPR. For the same signal acquisition time, the signal/noise ratio is >40 times higher for rapid-scan than for CW EPR. Rapid-scan EPR can detect superoxide produced by Enterococcus faecalis at rates that are too low for detection by CW EPR.
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