ArticleBiophysical journal2013
Unraveling the nanoscale surface properties of chitin synthase mutants of Aspergillus fumigatus and their biological implications.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Transcription factor RonA-driven GlcNAc catabolism is essential for growth, cell wall integrity, and pathogenicity inMicrobiology spectrum · 2025Article
- Comparative Analysis of the Aspergillus fumigatus Cell Wall Modification and Ensuing Human Dendritic Cell Responses by β-(1,3)-Glucan Synthase Inhibitors-Caspofungin and Enfumafungin.Mycopathologia · 2024Article
- Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus.Nature communications · 2024Article
- Solid-state NMR molecular snapshots ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Chitin Biosynthesis inJournal of fungi (Basel, Switzerland) · 2023Review
- Species-Specific Immunological Reactivities Depend on the Cell-Wall Organization of the TwoFrontiers in cellular and infection microbiology · 2021Article
- Deficiency of GPI Glycan Modification by Ethanolamine Phosphate Results in Increased Adhesion and Immune Resistance ofFrontiers in cellular and infection microbiology · 2021Article
- Differential Interactions of Serum and Bronchoalveolar Lavage Fluid Complement Proteins with Conidia of Airborne Fungal Pathogen Aspergillus fumigatus.Infection and immunity · 2020Article
- Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy.Cell surface (Amsterdam, Netherlands) · 2019Review
- Fungal melanin stimulates surfactant protein D-mediated opsonization of and host immune response toThe Journal of biological chemistry · 2018Article
- Atomic Force Microscopy: A Promising Tool for Deciphering the Pathogenic Mechanisms of Fungi in Cystic Fibrosis.Mycopathologia · 2018Review
- Article
- High-resolution imaging of the microbial cell surface.Journal of microbiology (Seoul, Korea) · 2016Review
- Single cell profiling of surface carbohydrates on Bacillus cereus.Journal of the Royal Society, Interface · 2015Article
- Surface structure characterization of Aspergillus fumigatus conidia mutated in the melanin synthesis pathway and their human cellular immune response.Infection and immunity · 2014Article
- Deletion of the α-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus.PLoS pathogens · 2013Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the surface properties of the human opportunistic pathogen Aspergillus fumigatus conidia is essential given the important role they play during the fungal interactions with the human host. Although chitin synthases with myosin motor-like domain (CSM) play a major role in cell wall biosynthesis, the extent to which deletion of the CSM genes alter the surface structural and biophysical-biological properties of conidia is not fully characterized. We used three complementary atomic force microscopy techniques-i.e., structural imaging, chemical force microscopy with hydrophobic tips, and single-molecule force spectroscopy with lectin tips-to gain detailed insights into the nanoscale surface properties (ultrastructure, hydrophobicity) and polysaccharide composition of the wild-type and the chitin synthase mutant (ΔcsmA, ΔcsmB, and ΔcsmA/csmB) conidia of A. fumigatus. Wild-type conidia were covered with a highly hydrophobic layer of rodlet nanostructures. By contrast, the surface of the ΔcsmA mutant was almost completely devoid of rodlets, leading to loss of hydrophobicity and exposure of mannan and chitin polysaccharides. The ΔcsmB and ΔcsmA/csmB mutants showed a different behavior, i.e., the surfaces featured poorly organized rodlet layers, yet with a low hydrophobicity and substantial amounts of exposed mannan and chitin at the surface. As the rodlet layer is important for masking recognition of immunogenic fungal cell wall components by innate immune cells, disappearance of rodlet layers in all three chitin synthase mutant conidia was associated with an activation of human dendritic cells. These nanoscale analyses emphasize the important and distinct roles that the CSMA and CSMB genes play in modulating the surface properties and immune interactions of A. fumigatus and demonstrate the power of atomic force microscopy in fungal genetic studies for assessing the phenotypic characteristics of mutants altered in cell surface organization.
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