ArticleSystems biology in reproductive medicine2013
Evaluation of the effectiveness of semen storage and sperm purification methods for spermatozoa transcript profiling.
Article in Systems biology in reproductive medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- On the state of chromatin in mammalian sperm.bioRxiv : the preprint server for biology · 2026Article
- Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.Advances in experimental medicine and biology · 2026Review
- Parental noncoding RNA expression dynamics across sperm, oocyte, and zygote.NAR genomics and bioinformatics · 2025Article
- Development and Optimization of an RNA-Isolating Protocol for Mammalian Spermatozoa.International journal of molecular sciences · 2025Article
- The composition of human sperm sncRNAome: a cross-country small RNA profiling.Reproductive biology and endocrinology : RB&E · 2025Article
- Tackling somatic DNA contamination in sperm epigenetic studies.Frontiers in reproductive health · 2025Article
- Impact of Supplemented Nutrition on Semen Quality, Epigenetic-Related Gene Expression, and Oxidative Status in Boars.Animals : an open access journal from MDPI · 2024Article
- Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm.Nature communications · 2021Article
- Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq.Frontiers in veterinary science · 2021Article
- What human sperm RNA-Seq tells us about the microbiome.Journal of assisted reproduction and genetics · 2020Article
- Single-cell sperm transcriptomes and variants from fathers of children with and without autism spectrum disorder.NPJ genomic medicine · 2020Article
- An Efficient Approach for RNA Extraction from Boar Sperm and Seminal Plasma.Bio-protocol · 2019Article
- Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application To Seminal Plasma From Infertile Patients.Molecular & cellular proteomics : MCP · 2019Article
- Spermatozoal large RNA content is associated with semen characteristics, sociodemographic and lifestyle factors.PloS one · 2019Article
- High-quality human and rat spermatozoal RNA isolation for functional genomic studies.Andrology · 2018Article
- Passing experiences on to future generations: endocrine disruptors and transgenerational inheritance of epimutations in brain and sperm.Epigenetics · 2018Article
- Subcellular localization and characterization of estrogenic pathway regulators and mediators in Atlantic salmon spermatozoal cells.Histochemistry and cell biology · 2018Article
- SpermBase: A Database for Sperm-Borne RNA Contents.Biology of reproduction · 2016Article
- Chromatin and extracellular vesicle associated sperm RNAs.Nucleic acids research · 2015Article
- Absence of sperm RNA elements correlates with idiopathic male infertility.Science translational medicine · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Different semen storage and sperm purification methods may affect the integrity of isolated spermatozoal RNA. RNA-Seq was applied to determine whether semen storage methods (pelleted vs. liquefied) and somatic cell lysis buffer (SCLB) vs. PureSperm (PS) purification methods affect the quantity and quality of sperm RNA. The results indicate that the method of semen storage does not markedly impact RNA profiling whereas the choice of purification can yield significant differences. RNA-Seq showed that the majority of mitochondrial and mid-piece associated transcripts were lost after SCLB purification, which indicated that the mid-piece of spermatozoa may have been compromised. In addition, the number of stable transcript pairs from SCLB-samples was less than that from the PS samples. This study supports the view that PS purification better maintains the integrity of spermatozoal RNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.