ArticlePlant cell reports2013
Defining reference genes for qPCR normalization to study biotic and abiotic stress responses in Vigna mungo.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 88 citations in OpenAlex.
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- NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.Scientific reports · 2024Article
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- Alternative splicing shapes the transcriptome complexity in blackgram [Vigna mungo (L.) Hepper].Functional & integrative genomics · 2023Article
- Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.Scientific reports · 2023Article
- Chitooligosaccharide Maintained Cell Membrane Integrity by Regulating Reactive Oxygen Species Homeostasis at Wounds of Potato Tubers during Healing.Antioxidants (Basel, Switzerland) · 2022Article
- Effects of Magnetite Nanoparticles and Static Magnetic Field on Neural Differentiation of Pluripotent Stem Cells.Stem cell reviews and reports · 2022Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Identification of HvLRX, a new dehydration and light responsive gene in Tibetan hulless barley (Hordeum vulgare var. nudum).Genes & genomics · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inInternational journal of molecular sciences · 2021Article
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- Hormonal and transcriptional analyses provides new insights into the molecular mechanisms underlying root thickening and isoflavonoid biosynthesis in Callerya speciosa (Champ. ex Benth.) Schot.Scientific reports · 2021Article
- Identification of Isoflavonoid Biosynthesis-Related R2R3-MYB Transcription Factors inInternational journal of genomics · 2021Article
- Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.Scientific reports · 2020Article
- Selection of Reference Genes for qRT-PCR Analysis in Medicinal PlantPlants (Basel, Switzerland) · 2020Article
- NO and ABA Interaction Regulates Tuber Dormancy and Sprouting in Potato.Frontiers in plant science · 2020Article
- Complex molecular mechanisms underlying MYMIV-resistance in Vigna mungo revealed by comparative transcriptome profiling.Scientific reports · 2019Article
- Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute.BMC molecular biology · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageExpression of ACT, EF1A; H2A, EF1A, ACT and 18S, TUB showed stability under MYMIV, salinity and drought stress, respectively; these are recommended as reference genes for qPCR normalization in Vigna mungo. Accurate gene expression profiling through qPCR depends on selection of appropriate reference gene(s) for normalization. Due to lack of unanimous internal standard, suitable constitutively expressed reference genes are selected that exhibit stable expression under diverse experimental conditions. In this communication, a comparative evaluation of stability among seven V. mungo genes encoding actin (ACT), histone H2A (H2A), elongation factor 1-alpha (EF1A), 18S rRNA (18S), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclophilin (CYP) and tubulin (TUB) under biotic (MYMIV) and abiotic (drought and salinity) stress conditions has been attempted. Specificity and amplification efficiency for each primer pair were verified; however, cumulative assessment of their accumulated transcripts revealed no uniformity. Therefore, individual stability and suitability of these seven candidates have been assessed in silico, by two widely used algorithms, geNorm and Normfinder. Based on the computed results, high stability was obtained for ACT and EF1A during MYMIV stress, while H2A, EFIA and ACT were found to be most suitable in salinity stress experiments and TUB and 18S during drought treatments. Combinations of ACT/TUB or ACT/EFIA were recommended for their use in the pooled analysis, while expression of 18S and CYP showed greater variations and therefore considered unsuitable as reference genes. Additionally, precise quantification of the target gene VmPRX under these stresses was shown to be a function of reference genes' stability, which tends to get affected when normalized with the least stable genes. Hence, use of these normalizers will facilitate accurate and reliable analyses of gene expression in V. mungo.
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