ArticleScientific reports2013
Two-photon voltage imaging using a genetically encoded voltage indicator.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Cross-species translational paradigms for assessing positive valence system as defined by the RDoC matrix.Journal of neurochemistry · 2025Review
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Review
- Discovering Microcircuit Secrets With Multi-Spot Imaging and Electrophysiological Recordings: The Example of Cerebellar Network Dynamics.Frontiers in cellular neuroscience · 2022Review
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Exciting Complexity: The Role of Motor Circuit Elements in ALS Pathophysiology.Frontiers in neuroscience · 2020Review
- Audio-visual experience strengthens multisensory assemblies in adult mouse visual cortex.Nature communications · 2019Article
- Peeking into the sleeping brain: Using in vivo imaging in rodents to understand the relationship between sleep and cognition.Journal of neuroscience methods · 2019Review
- Comparative Evaluation of Genetically Encoded Voltage Indicators.Cell reports · 2019Article
- Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning.PloS one · 2019Article
- Corrigendum: Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- Review
- Detection and analysis of spatiotemporal patterns in brain activity.PLoS computational biology · 2018Article
- Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.Brain structure & function · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-sensitive fluorescent proteins (VSFPs) are a family of genetically-encoded voltage indicators (GEVIs) reporting membrane voltage fluctuation from genetically-targeted cells in cell cultures to whole brains in awake mice as demonstrated earlier using 1-photon (1P) fluorescence excitation imaging. However, in-vivo 1P imaging captures optical signals only from superficial layers and does not optically resolve single neurons. Two-photon excitation (2P) imaging, on the other hand, has not yet been convincingly applied to GEVI experiments. Here we show that 2P imaging of VSFP Butterfly 1.2 expresssing pyramidal neurons in layer 2/3 reports optical membrane voltage in brain slices consistent with 1P imaging but with a 2-3 larger ΔR/R value. 2P imaging of mouse cortex in-vivo achieved cellular resolution throughout layer 2/3. In somatosensory cortex we recorded sensory responses to single whisker deflections in anesthetized mice at full frame video rate. Our results demonstrate the feasibility of GEVI-based functional 2P imaging in mouse cortex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.