ArticleMicrobial cell factories2013
Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- A Copper-Inducible Regulatory System for Dynamic Enhancement of Gene Editing Efficiency in Yarrowia lipolytica.Biotechnology journal · 2026Article
- Biosynthesis of conjugated linoleic acid: current status and future perspectives.Bioresources and bioprocessing · 2025Review
- Improving and Streamlining Gene Editing inJournal of fungi (Basel, Switzerland) · 2024Article
- Article
- A review of synthetic biology tools in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- EngineeringSynthetic and systems biotechnology · 2022Review
- The history, state of the art and future prospects for oleaginous yeast research.Microbial cell factories · 2021Review
- Metabolic Engineering for Unusual Lipid Production inMicroorganisms · 2020Review
- Review
- Modulating Heterologous Pathways and Optimizing Culture Conditions for Biosynthesis ofMolecules (Basel, Switzerland) · 2019Article
- The metabolism and genetic regulation of lipids in the oleaginous yeast Yarrowia lipolytica.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Review
- Synthetic biology tools for engineering Yarrowia lipolytica.Biotechnology advances · 2018Review
- Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-AccumulatingFrontiers in microbiology · 2018Article
- YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering inMetabolic engineering communications · 2017Article
- New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.Microbial cell factories · 2017Article
- A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica.Applied microbiology and biotechnology · 2017Article
- Lipid biosynthesis in yeasts: A comparison of the lipid biosynthetic pathway between the model nonoleaginous yeastEngineering in life sciences · 2017Review
- Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2015Article
- Engineering Ashbya gossypii for efficient biolipid production.Bioengineered · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConjugated linoleic acid (CLA) has been extensively studied for decades because of its health benefits including cancer prevention, anti-atherogenic and anti-obesity effects, and modulation of the immune system. We previously described the production of trans-10, cis-12 CLA in Yarrowia lipolytica by expressing the gene coding for linoleic acid isomerase from Propionibacterium acnes (pai). However the stable strain produced CLA at about 0.08% of dry cell weight (DCW), a level of production which was not high enough for practical applications. The goal of the present study was to enhance production of CLA by genetic engineering of Y. lipolytica strains.
resultsWe have now co-expressed the delta 12-desaturase gene (FADS12, d12) from Mortierella alpina together with the codon-optimized linoleic acid isomerase (opai) gene in Y. lipolytica, expressed under the control of promoter hp16d modified by fusing 12 copies of UAS1B to the original promoter hp4d. A multi-copy integration plasmid was used to further enhance the expression of both genes. Using glucose as the sole carbon source, the genetically-modified Y. lipolytica produced trans-10, cis-12-CLA at a level of up to 10% of total fatty acids and 0.4% of DCW. Furthermore, when the recombinant yeast was grown with soybean oil, trans-10, cis-12-CLA now accumulated at a level of up to 44% of total fatty acids, which represented 30% of DCW after 38.5 h of cultivation. In addition, trans-10, cis-12-CLA was also detected in the growth medium up to 0.9 g/l.
conclusionsWe have successfully produced trans-10, cis-12-CLA with a titre of 4 g/l of culture (3.1 g/l in cells and 0.9 g/l in culture medium). Our results demonstrate the potential use of Y. lipolytica as a promising microbial cell factory for trans-10, cis-12-CLA production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.