ArticleBMC microbiology2013
Ground Anthrax Bacillus Refined Isolation (GABRI) method for analyzing environmental samples with low levels of Bacillus anthracis contamination.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
- Synthetic peptide-based latex agglutination test for the detection ofRSC advances · 2026Article
- Hair and wool of ruminants and canines as environmental vectors of Bacillus anthracis and potential human exposure in anthrax-endemic regions.Scientific reports · 2026Article
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- Persistence in time: the hunt forApplied and environmental microbiology · 2025Article
- Evaluation of the Epidemic Situation of Anthrax in Armenia Over the Last Decade.Zoonoses and public health · 2025Article
- Archival and Newly Isolated HistoricalPathogens (Basel, Switzerland) · 2025Article
- EAVLD 2024 - 7Italian journal of food safety · 2024Article
- New Insights into the Phylogeny of the A.Br.161 ("A.Br.Heroin") Clade ofPathogens (Basel, Switzerland) · 2024Article
- One Health Assessment ofMicroorganisms · 2023Article
- Reoccurring Bovine Anthrax in Germany on the Same Pasture after 12 Years.Journal of clinical microbiology · 2022Article
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- Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations.Pathogens (Basel, Switzerland) · 2021Article
- Risk factors for bovine anthrax in Bangladesh, 2010-2014: a case-control study.Epidemiology and infection · 2020Article
- Epidemiological and comparative genomic analysis of Bacillus anthracis isolated from northern Vietnam.PloS one · 2020Article
- Colony-Forming Unit Spreadplate Assay versus Liquid Culture Enrichment-Polymerase Chain Reaction Assay for the Detection ofGeosciences · 2019Article
- Anthrax outbreaks in the humans - livestock and wildlife interface areas of Northern Tanzania: a retrospective record review 2006-2016.BMC public health · 2018Review
- Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): anthrax.EFSA journal. European Food Safety Authority · 2017Article
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9 authors.
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Abstract
backgroundIn this work are reported the results of a qualitative analytical method capable of detecting Bacillus anthracis spores when they are present in very low concentration in the soil. The Ground Anthrax Bacillus Refined Isolation (GABRI) method, assessed in our laboratory, was compared with the classic method. The comparison involved artificially anthrax-contaminated soil samples (500 spores/7.5 grams soil) and naturally contaminated soil samples collected in Bangladesh during a field investigation.
resultsThe results indicated that, in contrast to the classic method, the GABRI method was able to detect B.anthracis in all contaminated samples. The GABRI method produces a more sensitive measure of anthrax spore presence significantly different from the standard method. In particular, the latter is more sensitive to the presence of normal soil contaminants.
conclusionThe main feature of the GABRI method is its ability to strongly reduce the presence of the environmental contaminants, which being much more numerous than B. anthracis tend to inhibit their germination and growth making it extremely difficult to visualize any colonies. The reduction of the microbial environment also allows one to be able to culture and test a larger quantity of potentially contaminated soil and to isolate B. anthracis when the spores are present in very low concentrations in the soil.
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