ArticleBMC genomics2013
Distinct roles of the Gcn5 histone acetyltransferase revealed during transient stress-induced reprogramming of the genome.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- Integrative systems biology analysis of barley transcriptome ─ hormonal signaling against biotic stress.PloS one · 2023Pooled it
- PARylation of GCN5 by PARP1 mediates its recruitment to DSBs and facilitates both HR and NHEJ Repair.Cellular and molecular life sciences : CMLS · 2024Article
- Parallel Nonfunctionalization of CK1δ/ε Kinase Ohnologs Following a Whole-Genome Duplication Event.Molecular biology and evolution · 2023Article
- Parallel nonfunctionalization of CK1δ/ε kinase ohnologs following a whole-genome duplication event.bioRxiv : the preprint server for biology · 2023Article
- Functional tug of war between kinases, phosphatases, and the Gcn5 acetyltransferase in chromatin and cell cycle checkpoint controls.G3 (Bethesda, Md.) · 2023Article
- Functional Characterization of the GNAT Family Histone Acetyltransferase Elp3 and GcnE inInternational journal of molecular sciences · 2023Article
- Regulatory Roles of Histone Modifications in Filamentous Fungal Pathogens.Journal of fungi (Basel, Switzerland) · 2022Review
- Protein acetylation regulates xylose metabolism during adaptation of Saccharomyces cerevisiae.Biotechnology for biofuels · 2021Article
- USP22 SuppressesCancers · 2021Article
- Histone acetyltransferase PfGCN5 regulates stress responsive and artemisinin resistance related genes in Plasmodium falciparum.Scientific reports · 2021Article
- Transcription shapes genome-wide histone acetylation patterns.Nature communications · 2021Article
- Dynamic regulation of histone H3 lysine (K) acetylation and deacetylation during prolonged oxygen deprivation in a champion anaerobe.Molecular and cellular biochemistry · 2020Article
- USP22-dependent HSP90AB1 expression promotes resistance to HSP90 inhibition in mammary and colorectal cancer.Cell death & disease · 2019Article
- The Fungal Histone Acetyl Transferase Gcn5 Controls Virulence of the Human Pathogen Candida albicans through Multiple Pathways.Scientific reports · 2019Article
- Genome-Wide Detection of Genes Under Positive Selection in Worldwide Populations of the Barley Scald Pathogen.Genome biology and evolution · 2018Article
- TheFrontiers in microbiology · 2018Article
- Histone H3K4 and H3K36 Methylation Independently Recruit the NuA3 Histone Acetyltransferase inGenetics · 2017Article
- Nucleosome eviction in mitosis assists condensin loading and chromosome condensation.The EMBO journal · 2016Article
- H2A.Z marks antisense promoters and has positive effects on antisense transcript levels in budding yeast.BMC genomics · 2015Article
- Does gene length play a role? - Transient regulation of Gcn5 histone acetyltransferase under stress conditions.Genomics data · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGcn5 belongs to a family of histone acetyltransferases (HATs) that regulate protein function by acetylation. Gcn5 plays several different roles in gene transcription throughout the genome but their characterisation by classical mutation approaches is hampered by the high degree of apparent functional redundancy between HAT proteins.
resultsHere we utilise the reduced redundancy associated with the transiently high levels of genomic reprogramming during stress adaptation as a complementary approach to understand the functions of redundant protein families like HATs. We show genome-wide evidence for two functionally distinct roles of Gcn5. First, Gcn5 transiently re-localises to the ORFs of long genes during stress adaptation. Taken together with earlier mechanistic studies, our data suggests that Gcn5 plays a genome- wide role in specifically increasing the transcriptional elongation of long genes, thus increasing the production efficiency of complete long transcripts. Second, we suggest that Gcn5 transiently interacts with histones close to the transcription start site of the many genes that it activates during stress adaptation by acetylation of histone H3K18, leading to histone depletion, probably as a result of nucleosome loss as has been described previously.
conclusionsWe show that stress adaptation can be used to elucidate the functions of otherwise redundant proteins, like Gcn5, in gene transcription. Further, we show that normalization of chromatin-associated protein levels in ChIP experiments in relation to the histone levels may provide a useful complement to standard approaches. In the present study analysis of data in this way provides an alternative explanation for previously indicated repressive role of Gcn5 in gene transcription.
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