ArticleBioMed research international2013
Detergent-enzymatic decellularization of swine blood vessels: insight on mechanical properties for vascular tissue engineering.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Polyzwitterion-grafted decellularized bovine intercostal arteries as new substitutes of small-diameter arteries for vascular regeneration.Regenerative biomaterials · 2024Article
- Biomimetic tri-layered small-diameter vascular grafts with decellularized extracellular matrix promoting vascular regeneration and inhibiting thrombosis with the salidroside.Materials today. Bio · 2023Article
- Nerve regeneration using decellularized tissues: challenges and opportunities.Frontiers in neuroscience · 2023Review
- Small-diameter artery decellularization: Effects of anionic detergent concentration and treatment duration on porcine internal thoracic arteries.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Article
- Biochemical Aspects of Scaffolds for Cartilage Tissue Engineering; from Basic Science to Regenerative Medicine.The archives of bone and joint surgery · 2022Review
- Improving Biocompatibility of Polyester Fabrics through Polyurethane/Gelatin Complex Coating for Potential Vascular Application.Polymers · 2022Article
- Article
- Porcine carotid arteries decellularized with a suitable concentration combination of Triton X-100 and sodium dodecyl sulfate for tissue engineering vascular grafts.Cell and tissue banking · 2021Article
- Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold.Acta cirurgica brasileira · 2021Article
- Extracellular Matrix for Small-Diameter Vascular Grafts.Tissue engineering. Part A · 2020Review
- Antigen removal process preserves function of small diameter venous valved conduits, whereas SDS-decellularization results in significant valvular insufficiency.Acta biomaterialia · 2020Article
- Small Diameter Xenogeneic Extracellular Matrix Scaffolds for Vascular Applications.Tissue engineering. Part B, Reviews · 2020Review
- Polylysine Enriched Matrices: A Promising Approach for Vascular Grafts.Frontiers in bioengineering and biotechnology · 2020Article
- Suture retention strength of P(LLA-CL) tissue-engineered vascular grafts.RSC advances · 2019Article
- Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors.Nature communications · 2018Article
- Article
- Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering.Tissue engineering and regenerative medicine · 2017Article
- Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model.Biomedical engineering online · 2017Article
- Article
- Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications.BioMed research international · 2017Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small caliber vessels substitutes still remain an unmet clinical need; few autologous substitutes are available, while synthetic grafts show insufficient patency in the long term. Decellularization is the complete removal of all cellular and nuclear matters from a tissue while leaving a preserved extracellular matrix representing a promising tool for the generation of acellular scaffolds for tissue engineering, already used for various tissues with positive outcomes. The aim of this work is to investigate the effect of a detergent-enzymatic decellularization protocol on swine arteries in terms of cell removal, extracellular matrix preservation, and mechanical properties. Furthermore, the effect of storage at -80°C on the mechanical properties of the tissue is evaluated. Swine arteries were harvested, frozen, and decellularized; histological analysis revealed complete cell removal and preserved extracellular matrix. Furthermore, the residual DNA content in decellularized tissues was far low compared to native one. Mechanical testings were performed on native, defrozen, and decellularized tissues; no statistically significant differences were reported for Young's modulus, ultimate stress, compliance, burst pressure, and suture retention strength, while ultimate strain and stress relaxation of decellularized vessels were significantly different from the native ones. Considering the overall results, the process was confirmed to be suitable for the generation of acellular scaffolds for vascular tissue engineering.
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