ArticleThe Korean journal of parasitology2013
Cryptosporidium hominis infection diagnosed by real-time PCR-RFLP.
Article in The Korean journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- Review
- Causes of acute gastroenteritis in Korean children between 2004 and 2019.Clinical and experimental pediatrics · 2021Article
- Lateral Flow Loop-Mediated Isothermal Amplification Test with Stem Primers: Detection ofJournal of tropical medicine · 2018Article
- Detection of Cryptosporidium parvum in environmental soil and vegetables.Journal of Korean medical science · 2014Article
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7 authors.
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Abstract
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
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