ArticleThe Korean journal of parasitology2013
Serodiagnosis of echinococcosis by ELISA using cystic fluid from Uzbekistan sheep.
Article in The Korean journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- Enhancing hydatid cysts diagnosis utilizing cell-free DNA as a sensitive biomarker forHelminthologia · 2026Article
- Delayed Diagnosis of Imported Cystic Echinococcosis and Successful Treatment With Percutaneous Drainage and Albendazole in Korea: A Case Report.Journal of Korean medical science · 2025Article
- Human and camel cystic echinococcosis - a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification.Veterinary research communications · 2024Article
- A Novel Designed Sandwich ELISA for the Detection ofTropical medicine and infectious disease · 2023Article
- Modelling diagnostics forOne health (Amsterdam, Netherlands) · 2022Article
- A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis.Veterinary world · 2020Article
- An Imported Case of Disseminated Echinococcosis in Korea.The Korean journal of parasitology · 2019Article
- Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep.The Korean journal of parasitology · 2019Article
- Presumptive case of sparganosis manifesting as a hepatic mass: A case report and literature review.World journal of radiology · 2016Article
- Immunodiagnosis of human hydatid disease: Where do we stand?World journal of methodology · 2015Review
- Prevalence of Serum IgG Antibodies to Cystic Echinococcus Antigen among Patients in an Uzbekistan Emergency Hospital.The Korean journal of parasitology · 2015Article
- Infection status of hydatid cysts in humans and sheep in Uzbekistan.The Korean journal of parasitology · 2013Article
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5 authors.
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Abstract
According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases.
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