ArticleMolecular biology of the cell2013
The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice.Cellular and molecular life sciences : CMLS · 2026Article
- ELAPOR1 regulates VPS54-mediated GARP complex formation and proacrosomal vesicle fusion during spermatogenesis.Theranostics · 2026Article
- USO1 Coordinates Centriolar Satellites to Regulate Male Germ Cell Proliferation and Cell Cycle Progression.International journal of molecular sciences · 2025Article
- Bovine Spermatogenesis.Advances in anatomy, embryology, and cell biology · 2025Review
- Sperm acrosome overgrowth and infertility in mice lacking chromosome 18 pachytene piRNA.PLoS genetics · 2021Article
- Genome-Wide Association Study-Based Identification of SNPs and Haplotypes Associated With Goose Reproductive Performance and Egg Quality.Frontiers in genetics · 2021Article
- AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia.Frontiers in cell and developmental biology · 2021Article
- Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility.BMC veterinary research · 2020Article
- Sperm Differentiation: The Role of Trafficking of Proteins.International journal of molecular sciences · 2020Review
- Vps13b is required for acrosome biogenesis through functions in Golgi dynamic and membrane trafficking.Cellular and molecular life sciences : CMLS · 2020Article
- Abnormal fertility, acrosome formation, IFT20 expression and localization in conditionalAmerican journal of physiology. Cell physiology · 2020Article
- Proteomic alterations underlie an association with teratozoospermia in obese mice sperm.Reproductive biology and endocrinology : RB&E · 2019Article
- Mechanism of Acrosome Biogenesis in Mammals.Frontiers in cell and developmental biology · 2019Review
- AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice.Scientific reports · 2018Article
- Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models.International journal of molecular sciences · 2017Article
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- The control of male fertility by spermatid-specific factors: searching for contraceptive targets from spermatozoon's head to tail.Cell death & disease · 2016Review
- Article
- Accumulation of glucosylceramide in the absence of the beta-glucosidase GBA2 alters cytoskeletal dynamics.PLoS genetics · 2015Article
- Potential Gene Interactions in the Cell Cycles of Gametes, Zygotes, Embryonic Stem Cells and the Development of Cancer.Frontiers in oncology · 2015Review
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Authors and funding
23 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The trans-Golgi network (TGN) functions as a hub organelle in the exocytosis of clathrin-coated membrane vesicles, and SMAP2 is an Arf GTPase-activating protein that binds to both clathrin and the clathrin assembly protein (CALM). In the present study, SMAP2 is detected on the TGN in the pachytene spermatocyte to the round spermatid stages of spermatogenesis. Gene targeting reveals that SMAP2-deficient male mice are healthy and survive to adulthood but are infertile and exhibit globozoospermia. In SMAP2-deficient spermatids, the diameter of proacrosomal vesicles budding from TGN increases, TGN structures are distorted, acrosome formation is severely impaired, and reorganization of the nucleus does not proceed properly. CALM functions to regulate vesicle sizes, and this study shows that CALM is not recruited to the TGN in the absence of SMAP2. Furthermore, syntaxin2, a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, is not properly concentrated at the site of acrosome formation. Thus this study reveals a link between SMAP2 and CALM/syntaxin2 in clathrin-coated vesicle formation from the TGN and subsequent acrosome formation. SMAP2-deficient mice provide a model for globozoospermia in humans.
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