ArticleMolecular biology of the cell2013
Sit4p/PP6 regulates ER-to-Golgi traffic by controlling the dephosphorylation of COPII coat subunits.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites.Nature communications · 2025Article
- Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications.Nucleic acids research · 2025Article
- Substrate displacement of CK1 C-termini regulates kinase specificity.Science advances · 2024Article
- Sit4 Genetically Interacts with Vps27 to Regulate Mitochondrial Function and Lifespan inCells · 2024Article
- The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence.PLoS pathogens · 2023Article
- The phosphatase Glc7 controls the eisosomal response to starvation via post-translational modification of Pil1.Journal of cell science · 2023Article
- The C-terminus of the cargo receptor Erv14 affects COPII vesicle formation and cargo delivery.Journal of cell science · 2023Article
- The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast.International journal of molecular sciences · 2021Article
- Article
- The AGCVIII kinase Dw2 modulates cell proliferation, endomembrane trafficking, and MLG/xylan cell wall localization in elongating stem internodes of Sorghum bicolor.The Plant journal : for cell and molecular biology · 2021Article
- Methods for Assessing the Regulation of a Kinase by the Rab GTPase Ypt1.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Export Control: Post-transcriptional Regulation of the COPII Trafficking Pathway.Frontiers in cell and developmental biology · 2020Review
- A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation.Science (New York, N.Y.) · 2019Article
- COPII-dependent ER export in animal cells: adaptation and control for diverse cargo.Histochemistry and cell biology · 2018Review
- Article
- The ceramide activated protein phosphatase Sit4 impairs sphingolipid dynamics, mitochondrial function and lifespan in a yeast model of Niemann-Pick type C1.Biochimica et biophysica acta. Molecular basis of disease · 2018Article
- MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity.Science signaling · 2017Article
- Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Traffic from the endoplasmic reticulum (ER) to the Golgi complex is initiated when the activated form of the GTPase Sar1p recruits the Sec23p-Sec24p complex to ER membranes. The Sec23p-Sec24p complex, which forms the inner shell of the COPII coat, sorts cargo into ER-derived vesicles. The coat inner shell recruits the Sec13p-Sec31p complex, leading to coat polymerization and vesicle budding. Recent studies revealed that the Sec23p subunit sequentially interacts with three different binding partners to direct a COPII vesicle to the Golgi. One of these binding partners is the serine/threonine kinase Hrr25p. Hrr25p phosphorylates the COPII coat, driving the membrane-bound pool into the cytosol. The phosphorylated coat cannot rebind to the ER to initiate a new round of vesicle budding unless it is dephosphorylated. Here we screen all known protein phosphatases in yeast to identify one whose loss of function alters the cellular distribution of COPII coat subunits. This screen identifies the PP2A-like phosphatase Sit4p as a regulator of COPII coat dephosphorylation. Hyperphosphorylated coat subunits accumulate in the sit4Δ mutant in vivo. In vitro, Sit4p dephosphorylates COPII coat subunits. Consistent with a role in coat recycling, Sit4p and its mammalian orthologue, PP6, regulate traffic from the ER to the Golgi complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.