ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Expression of the γ2-subunit distinguishes synaptic and extrasynaptic GABA(A) receptors in NG2 cells of the hippocampus.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
- Prefrontal gamma oscillations and fear extinction learning require early postnatal interneuron-oligodendroglia communication.Nature communications · 2025Article
- Interaction between Oligodendrocytes and Interneurons in Brain Development and Related Neuropsychiatric Disorders.International journal of molecular sciences · 2024Review
- Impact of Developmental Changes of GABAInternational journal of molecular sciences · 2023Article
- Transcriptional and bioinformatic analysis of GABAFrontiers in molecular neuroscience · 2023Article
- Impaired bidirectional communication between interneurons and oligodendrocyte precursor cells affects social cognitive behavior.Nature communications · 2022Article
- The bright and the dark side of myelin plasticity: Neuron-glial interactions in health and disease.Seminars in cell & developmental biology · 2021Article
- Oligodendroglial GABAergic Signaling: More Than Inhibition!Neuroscience bulletin · 2021Review
- Building a (w)rapport between neurons and oligodendroglia: Reciprocal interactions underlying adaptive myelination.Neuron · 2021Review
- GABAMolecular pharmacology · 2021Article
- Therapeutic Potential of GABAergic Signaling in Myelin Plasticity and Repair.Frontiers in cell and developmental biology · 2021Review
- Novel Toolboxes for the Investigation of Activity-Dependent Myelination in the Central Nervous System.Frontiers in cellular neuroscience · 2021Review
- Neuron to Oligodendrocyte Precursor Cell Synapses: Protagonists in Oligodendrocyte Development and Myelination, and Targets for Therapeutics.Frontiers in neuroscience · 2021Review
- The roles of neuron-NG2 glia synapses in promoting oligodendrocyte development and remyelination.Cell and tissue research · 2020Review
- Morphological and immunophenotypic characterization of perivascular interstitial cells in human glioma: Telocytes, pericytes, and mixed immunophenotypes.Oncotarget · 2020Article
- Expression and Function of GABA Receptors in Myelinating Cells.Frontiers in cellular neuroscience · 2020Article
- Article
- Agarwood Essential Oil Displays Sedative-Hypnotic Effects through the GABAergic System.Molecules (Basel, Switzerland) · 2017Article
- Electrophysiological properties of NG2(+) cells: Matching physiological studies with gene expression profiles.Brain research · 2016Review
- Physiological impact of CB1 receptor expression by hippocampal GABAergic interneurons.Pflugers Archiv : European journal of physiology · 2016Article
- Review
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6 authors.
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Abstract
NG2 cells are equipped with transmitter receptors and receive direct synaptic input from glutamatergic and GABAergic neurons. The functional impact of these neuron-glia synapses is still unclear. Here, we combined functional and molecular techniques to analyze properties of GABA(A) receptors in NG2 cells of the juvenile mouse hippocampus. GABA activated slowly desensitizing responses in NG2 cells, which were mimicked by muscimol and inhibited by bicuculline. To elucidate the subunit composition of the receptors we tested its pharmacological properties. Coapplication of pentobarbital, benzodiazepines, and zolpidem all significantly increased the GABA-evoked responses. The presence of small tonic currents indicated the presence of extrasynaptic GABA(A) receptors. To further analyze the subunit expression, single cell transcript analysis was performed subsequent to functional characterization of NG2 cells. The subunits α1, α2, β3, γ1, and γ2 were most abundantly expressed, matching properties resulting from pharmacological characterization. Importantly, lack of the γ2-subunit conferred a high Zn²⁺ sensitivity to the GABA(A) receptors of NG2 cells. Judging from the zolpidem sensitivity, postsynaptic GABA(A) receptors in NG2 cells contain the γ2-subunit, in contrast to extrasynaptic receptors, which were not modulated by zolpidem. To determine the effect of GABA(A) receptor activation on membrane potential, perforated patch recordings were obtained from NG2 cells. In the current-clamp mode, GABA depolarized the cells to approximately -30 mV, indicating a higher intracellular Cl⁻ concentration (∼50 mM) than previously reported. GABA-induced depolarization in NG2 cells might trigger Ca²⁺ influx through voltage-activated Ca²⁺ channels.
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