ArticleIrish journal of medical science2014
Early diagnosis of tuberculous meningitis by an indirect ELISA protocol based on the detection of the antigen ESAT-6 in cerebrospinal fluid.
Article in Irish journal of medical science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Aqueous extract-mediated green synthesis of CuO nanoparticles: Potential anti-tuberculosis agents.Food science & nutrition · 2024Article
- Xpert MTB/RIF Ultra for the Diagnosis of Tuberculous Meningitis: A Small Step Forward.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Role of Immuno-Polymerase Chain Reaction (I-PCR) in Resolving Diagnostic Dilemma Between Tuberculoma and Neurocysticercosis: A Case Report.The American journal of case reports · 2018Article
- Biomarkers of Cerebral Injury and Inflammation in Pediatric Tuberculous Meningitis.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Comparison of the Performance of UrinaryInternational journal of microbiology · 2017Article
- Laboratory Diagnosis of Central Nervous System Infection.Current infectious disease reports · 2016Review
- Roles of mycobacterium tuberculosis ESAT-6 in the development of renal injury.International journal of clinical and experimental medicine · 2015Article
- Detection of pandemic strain of influenza virus (A/H1N1/pdm09) in pigs, West Africa: implications and considerations for prevention of future influenza pandemics at the source.Infection ecology & epidemiology · 2015Article
- pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.PloS one · 2014Article
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5 authors at 1 institution in 1 country.
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Abstract
backgroundEarly diagnosis of tuberculous meningitis (TBM) is still a challenge; the present study aimed to establish a method of detecting the antigen early secreted antigenic target 6 (ESAT-6) in cerebrospinal fluid (CSF) by an indirect enzyme-linked immunosorbance assay (ELISA) protocol and to study the value of detecting ESAT-6 in CSF in the early diagnosis of TBM.
methodsAn indirect ELISA protocol was used, employing a monoclonal antibody (mAb) against ESAT-6, which was used to demonstrate ESAT-6 in the CSF from TBM patients and non-TBM controls. CSF was obtained from 100 patients: confirmed TBM, clinically diagnosed TBM, disease controls, and healthy controls (n = 10). Pure recombinant ESAT-6 (standard product) was used in serial dilutions to detect the absorbance and to establish a standard curve from the data; the concentration was on the X axis vs. absorbance on the Y axis, and the standard curve was used to interpolate the concentration of ESAT-6 in samples.
resultsThe indirect ELISA method provided 88 % sensitivity and 92 % specificity for the diagnosis of TBM using a mAb to ESAT-6. The mean concentration of ESAT-6 in TBM patients was significantly higher than that of the non-TBM groups. There was also a significant difference in the mean ESAT-6 expression between the confirmed TBM patients and the clinically diagnosed TBM patients (p < 0.01).
conclusionsDetection of ESAT-6 in the CSF of TBM patients by indirect ELISA protocol gives a reliable early diagnosis and can be used to develop an immunodiagnostic assay with increased sensitivity and specificity.
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