ArticleThe Journal of bone and joint surgery. American volume2013
Wnt3a reestablishes osteogenic capacity to bone grafts from aged animals.
Article in The Journal of bone and joint surgery. American volume, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Wnt/β-catenin signaling pathway as an important mediator in muscle and bone crosstalk: A systematic review.Journal of orthopaedic translation · 2024Review
- Cellular senescence in skeletal disease: mechanisms and treatment.Cellular & molecular biology letters · 2023Review
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- Therapeutic approaches to activate the canonical Wnt pathway for bone regeneration.Journal of tissue engineering and regenerative medicine · 2022Review
- Hormone sensitive lipase ablation promotes bone regeneration.Biochimica et biophysica acta. Molecular basis of disease · 2022Article
- Carbon Monoxide-Releasing Molecule-3 Enhances Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells via miR-195-5p/Wnt3a Pathway.Drug design, development and therapy · 2022Article
- Systemic NF-κB-mediated inflammation promotes an aging phenotype in skeletal stem/progenitor cells.Aging · 2021Article
- Optimizing autologous bone contribution to implant osseointegration.Journal of periodontology · 2020Article
- Ankylosis progressive homolog upregulation inhibits cell viability and mineralization during fibroblast ossification by regulating the Wnt/β‑catenin signaling pathway.Molecular medicine reports · 2020Article
- Effect of Collagen Cross-Link Deficiency on Incorporation of Grafted Bone.Dentistry journal · 2019Article
- Expanding the phenome and variome of skeletal dysplasia.Genetics in medicine : official journal of the American College of Medical Genetics · 2018Article
- Article
- Masquelet technique: The effect of altering implant material and topography on membrane matrix composition, mechanical and barrier properties in a rat defect model.Journal of biomechanics · 2018Article
- A WNT protein therapeutic improves the bone-forming capacity of autografts from aged animals.Scientific reports · 2018Article
- Microenvironmental Regulation of Chondrocyte Plasticity in Endochondral Repair-A New Frontier for Developmental Engineering.Frontiers in bioengineering and biotechnology · 2018Review
- Bone healing in an aged murine fracture model is characterized by sustained callus inflammation and decreased cell proliferation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- WNT-activated bone grafts repair osteonecrotic lesions in aged animals.Scientific reports · 2017Article
- Ecto-5'-nucleotidase (CD73) regulates bone formation and remodeling during intramembranous bone repair in aging mice.Tissue & cell · 2017Article
- Biophysical regulation of osteotomy healing: An animal study.Clinical implant dentistry and related research · 2017Article
- Relationships among Bone Quality, Implant Osseointegration, and Wnt Signaling.Journal of dental research · 2017Article
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Authors and funding
13 authors at 6 institutions in 1 country.
Funding
Abstract
backgroundAge-related fatty degeneration of the bone marrow contributes to delayed fracture-healing and osteoporosis-related fractures in the elderly. The mechanisms underlying this fatty change are unknown, but they may relate to the level of Wnt signaling within the aged marrow cavity.
methodsTransgenic mice were used in conjunction with a syngeneic bone-graft model to follow the fates of cells involved in the engraftment. Immunohistochemistry along with quantitative assays were used to evaluate Wnt signaling and adipogenic and osteogenic gene expression in bone grafts from young and aged mice. Liposomal Wnt3a protein (L-Wnt3a) was tested for its ability to restore osteogenic potential to aged bone grafts in critical-size defect models created in mice and in rabbits. Radiography, microquantitative computed tomography (micro-CT) reconstruction, histology, and histomorphometric measurements were used to quantify bone-healing resulting from L-Wnt3a or a control substance (liposomal phosphate-buffered saline solution [L-PBS]).
resultsExpression profiling of cells in a bone graft demonstrated a shift away from an osteogenic gene profile and toward an adipogenic one with age. This age-related adipogenic shift was accompanied by a significant reduction (p < 0.05) in Wnt signaling and a loss in osteogenic potential. In both large and small animal models, osteogenic competence was restored to aged bone grafts by a brief incubation with the stem-cell factor Wnt3a. In addition, liposomal Wnt3a significantly reduced cell death in the bone graft, resulting in significantly more osseous regenerate in comparison with controls.
conclusionsLiposomal Wnt3a enhances cell survival and reestablishes the osteogenic capacity of bone grafts from aged animals. CLINICAL RELEVANCE: We developed an effective, clinically applicable, regenerative medicine-based strategy for revitalizing bone grafts from aged patients.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.