ArticleNucleic acids research2013
Controlling the stoichiometry and strand polarity of a tetramolecular G-quadruplex structure by using a DNA origami frame.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 49 citations in OpenAlex.
- A Facile and Reproducible Method for the Purification of Peptide- and Protein-Functionalized DNA Nanostructures.JACS Au · 2025Article
- Structural characterization of humanFrontiers in oncology · 2023Article
- DNA Manipulation and Single-Molecule Imaging.Molecules (Basel, Switzerland) · 2021Review
- Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability.Molecules (Basel, Switzerland) · 2019Article
- Molecular-scale visualization and surface charge density measurement of Z-DNA in aqueous solution.Scientific reports · 2019Article
- Impact of Small Molecules on Intermolecular G-Quadruplex Formation.Molecules (Basel, Switzerland) · 2019Article
- DNA nanostructures: A versatile lab-bench for interrogating biological reactions.Computational and structural biotechnology journal · 2019Review
- Structure and conformational dynamics of scaffolded DNA origami nanoparticles.Nucleic acids research · 2017Article
- Structure, Properties, and Biological Relevance of the DNA and RNA G-Quadruplexes: Overview 50 Years after Their Discovery.Biochemistry. Biokhimiia · 2016Article
- Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure.Nucleic acids research · 2014Article
- HIV-1 nucleocapsid proteins as molecular chaperones for tetramolecular antiparallel G-quadruplex formation.Journal of the American Chemical Society · 2013Article
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Authors and funding
6 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Guanine-rich oligonucleotides often show a strong tendency to form supramolecular architecture, the so-called G-quadruplex structure. Because of the biological significance, it is now considered to be one of the most important conformations of DNA. Here, we describe the direct visualization and single-molecule analysis of the formation of a tetramolecular G-quadruplex in KCl solution. The conformational changes were carried out by incorporating two duplex DNAs, with G-G mismatch repeats in the middle, inside a DNA origami frame and monitoring the topology change of the strands. In the absence of KCl, incorporated duplexes had no interaction and laid parallel to each other. Addition of KCl induced the formation of a G-quadruplex structure by stably binding the duplexes to each other in the middle. Such a quadruplex formation allowed the DNA synapsis without disturbing the duplex regions of the participating sequences, and resulted in an X-shaped structure that was monitored by atomic force microscopy. Further, the G-quadruplex formation in KCl solution and its disruption in KCl-free buffer were analyzed in real-time. The orientation of the G-quadruplex is often difficult to control and investigate using traditional biochemical methods. However, our method using DNA origami could successfully control the strand orientations, topology and stoichiometry of the G-quadruplex.
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