ArticleNature communications2013
Fast GCaMPs for improved tracking of neuronal activity.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed.
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Calcium Buffering in Astrocytes and Its Relevance for Experimental Data Interpretation and Computational Modeling.Journal of neurochemistry · 2026Review
- Serotonergic Signaling GovernseNeuro · 2025Article
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Precise calcium-to-spike inference using biophysical generative models.Research square · 2025Article
- Recent odor experience selectively modulates olfactory sensitivity across the glomerular output in the mouse olfactory bulb.Chemical senses · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Recent odor experience selectively modulates olfactory sensitivity across the glomerular output in the mouse olfactory bulb.bioRxiv : the preprint server for biology · 2024Article
- A pupillary contrast response in mice and humans: Neural mechanisms and visual functions.Neuron · 2024Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant.Communications biology · 2024Article
- Exploring CaeNeuro · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Localized calcium transients in phragmoplast regulate cytokinesis of tobacco BY-2 cells.Plant cell reports · 2024Article
- Structural and Functional Organization of Visual Responses in the Inferior Olive of Larval Zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells.Nature methods · 2023Article
- Direct comparison reveals algorithmic similarities in fly and mouse visual motion detection.iScience · 2023Article
- Highly specific and non-invasive imaging of Piezo1-dependent activity across scales using GenEPi.Nature communications · 2023Article
- Transneuronal Circuit Analysis with Pseudorabies Viruses.Current protocols · 2023Review
- Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
The use of genetically encodable calcium indicator proteins to monitor neuronal activity is hampered by slow response times and a narrow Ca(2+)-sensitive range. Here we identify three performance-limiting features of GCaMP3, a popular genetically encodable calcium indicator protein. First, we find that affinity is regulated by the calmodulin domain's Ca(2+)-chelating residues. Second, we find that off-responses to Ca(2+) are rate-limited by dissociation of the RS20 domain from calmodulin's hydrophobic pocket. Third, we find that on-responses are limited by fast binding to the N-lobe at high Ca(2+) and by slow binding to the C-lobe at lower Ca(2+). We develop Fast-GCaMPs, which have up to 20-fold accelerated off-responses and show that they have a 200-fold range of K(D), allowing coexpression of multiple variants to span an expanded range of Ca(2+) concentrations. Finally, we show that Fast-GCaMPs track natural song in Drosophila auditory neurons and generate rapid responses in mammalian neurons, supporting the utility of our approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.