ArticleBiology of reproduction2013
A requirement for fatty acid oxidation in the hormone-induced meiotic maturation of mouse oocytes.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Age-associated changes in cumulus cells and follicular fluid: the local oocyte microenvironment as a determinant of gamete quality.Biology of reproduction · 2022Pooled it
- Intrafollicular cortisol levels inversely correlate with cumulus cell lipid content as a possible energy source during oocyte meiotic resumption in women undergoing ovarian stimulation for in vitro fertilization.Fertility and sterility · 2015Trial
- Cumulus Expansion and Oocyte Quality Across the Pubertal Transition in Mice: Implications for Modeling Fertility Preservation in Adolescents.Research square · 2026Article
- The role of metabolic reprogramming in oocyte development: research progress and prospects.Frontiers in reproductive health · 2026Review
- Fatty acid metabolism of granulose cells and follicular fluid and their associations with embryo quality in normal-weight women with PCOS.Journal of ovarian research · 2025Article
- Ovarian aging: energy metabolism of oocytes.Journal of ovarian research · 2024Review
- The oocyte microenvironment is altered in adolescents compared to oocyte donors.Human reproduction open · 2024Article
- Ectopic Lipid Accumulation Correlates with Cellular Stress in Rabbit Blastocysts from Diabetic Mothers.International journal of molecular sciences · 2023Article
- Gestational Benzo[a]pyrene Exposure Destroys F1 Ovarian Germ Cells Through Mitochondrial Apoptosis Pathway and Diminishes Surviving Oocyte Quality.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Glutathione deficiency decreases lipid droplet stores and increases reactive oxygen species in mouse oocytes†.Biology of reproduction · 2022Article
- Reduction of oocyte lipid droplets and meiotic failure due to biotin deficiency was not rescued by restoring the biotin nutritional status.Nutrition research and practice · 2022Article
- Carnitines as Mitochondrial Modulators of Oocyte and Embryo Bioenergetics.Antioxidants (Basel, Switzerland) · 2022Review
- Porcine bile acids promote the utilization of fat and vitamin A under low-fat diets.Frontiers in nutrition · 2022Article
- Lipid Metabolic Process Involved in Oocyte Maturation During Folliculogenesis.Frontiers in cell and developmental biology · 2022Review
- Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation.Cell stem cell · 2020Article
- Review
- Proteomics Recapitulates Ovarian Proteins Relevant to Puberty and Fertility in Brahman Heifers (Genes · 2019Article
- Fatty Acid β-Oxidation Is Essential in Leptin-Mediated Oocytes Maturation of Yellow Catfish Pelteobagrus fulvidraco.International journal of molecular sciences · 2018Article
- Aberrant expression and DNA methylation of lipid metabolism genes in PCOS: a new insight into its pathogenesis.Clinical epigenetics · 2018Article
- Effects of Mild and Severe Vitamin BNutrition and metabolic insights · 2017Article
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2 authors.
Funding
Abstract
We have previously shown that fatty acid oxidation (FAO) is required for AMP-activated protein kinase (PRKA)-induced maturation in vitro. In the present study, we have further investigated the role of this metabolic pathway in hormone-induced meiotic maturation. Incorporating an assay with (3)H-palmitic acid as the substrate, we first examined the effect of PRKA activators on FAO levels. There was a significant stimulation of FAO in cumulus cell-enclosed oocytes (CEO) treated with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) and RSVA405. In denuded oocytes (DO), AICAR stimulated FAO only in the presence of carnitine, the molecule that facilitates fatty acyl CoA entry into the mitochondria. The carnitine palmitoyltransferase 1 activator C75 successfully stimulated FAO in CEO. All three of these activators trigger germinal vesicle breakdown. Meiotic resumption induced by follicle-stimulating hormone (FSH) or amphiregulin was completely inhibited by the FAO inhibitors etomoxir, mercaptoacetate, and malonyl CoA. Importantly, FAO was increased in CEO stimulated by FSH and epidermal growth factor, and this increase was blocked by FAO inhibitors. Moreover, compound C, a PRKA inhibitor, prevented the FSH-induced increase in FAO. Both carnitine and palmitic acid augmented hormonal induction of maturation. In a more physiological setting, etomoxir eliminated human chorionic gonadotropin (hCG)-induced maturation in follicle-enclosed oocytes. In addition, CEO and DO from hCG-treated mice displayed an etomoxir-sensitive increase in FAO, indicating that this pathway was stimulated during in vivo meiotic resumption. Taken together, our data indicate that hormone-induced maturation in mice requires a PRKA-dependent increase in FAO.
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