ArticlePloS one2013
A reporter screen in a human haploid cell line identifies CYLD as a constitutive inhibitor of NF-κB.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- The role of e3 ubiquitin ligases and deubiquitinating enzymes in hepatocellular carcinoma.Cell biology and toxicology · 2026Review
- Genomic Characterization of Prostatic Basal Cell Carcinoma.The American journal of pathology · 2023Article
- A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.mBio · 2021Article
- Article
- A genome-wide CRISPR screen identifies regulation factors of the TLR3 signalling pathway.Innate immunity · 2020Article
- A genome-wide enrichment screen identifies NUMA1-loss as a resistance mechanism against mitotic cell-death induced by BMI1 inhibition.PloS one · 2020Article
- Integrin activation by the lipid molecule 25-hydroxycholesterol induces a proinflammatory response.Nature communications · 2019Article
- A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel.The Journal of general physiology · 2019Review
- Keeping Cell Death in Check: Ubiquitylation-Dependent Control of TNFR1 and TLR Signaling.Frontiers in cell and developmental biology · 2019Article
- New tools for old drugs: Functional genetic screens to optimize current chemotherapy.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2018Review
- HaSAPPy: A tool for candidate identification in pooled forward genetic screens of haploid mammalian cells.PLoS computational biology · 2018Article
- RNAi-Based Identification of Gene-Specific Nuclear Cofactor Networks Regulating Interleukin-1 Target Genes.Frontiers in immunology · 2018Article
- SPATA2: more than a missing link.Cell death and differentiation · 2017Review
- Identification of Genes That Modulate Susceptibility to Formaldehyde and Imatinib by Functional Genomic Screening in Human Haploid KBM7 Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Large-Scale Single Guide RNA Library Construction and Use for CRISPR-Cas9-Based Genetic Screens.Cold Spring Harbor protocols · 2016Article
- Recombinase-based conditional and reversible gene regulation via XTR alleles.Nature communications · 2015Article
- Hunting Viral Receptors Using Haploid Cells.Annual review of virology · 2015Review
- Gene transfer and genome-wide insertional mutagenesis by retroviral transduction in fish stem cells.PloS one · 2015Article
- The protein synthesis inhibitor blasticidin s enters mammalian cells via leucine-rich repeat-containing protein 8D.The Journal of biological chemistry · 2014Article
- Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9.Mutation research. Reviews in mutation researchReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. So far, this approach has been limited mostly to the identification of genes that mediate cell death in response to a lethal agent, likely due to the ease with which this phenotype can be observed. Here, we perform the first reporter screen in the near-haploid KBM7 cell line to identify constitutive inhibitors of NF-κB. CYLD was the only currently known negative regulator of NF-κB to be identified, thus uniquely distinguishing this gene. Also identified were three genes with no previous known connection to NF-κB. Our results demonstrate that reporter screens in haploid human cells can be applied to investigate the many complex signaling pathways that converge upon transcription factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.