ArticlePloS one2013
Isospora suis in an epithelial cell culture system - an in vitro model for sexual development in coccidia.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Unravelling the sexual developmental biology ofFrontiers in cellular and infection microbiology · 2023Article
- The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia).Scientific reports · 2022Article
- Editorial: Get Over the Gut: Apicomplexan Parasite Interaction, Survival and Stage Progression in Vertebrate and Invertebrate Digestive Tracts.Frontiers in cellular and infection microbiology · 2021Article
- Reduced treatment frequencies with bumped kinase inhibitor 1369 are effective against porcine cystoisosporosis.International journal for parasitology. Drugs and drug resistance · 2020Article
- Characterization of Cystoisospora suis sexual stages in vitro.Parasites & vectors · 2020Article
- The apical annuli of Toxoplasma gondii are composed of coiled-coil and signalling proteins embedded in the inner membrane complex sutures.Cellular microbiology · 2020Article
- Fussing About Fission: Defining Variety Among Mainstream and Exotic Apicomplexan Cell Division Modes.Frontiers in cellular and infection microbiology · 2020Article
- Bumped kinase inhibitor 1369 is effective against Cystoisospora suis in vivo and in vitro.International journal for parasitology. Drugs and drug resistance · 2019Article
- Microsatellite Analysis of Geographically Close Isolates ofFrontiers in veterinary science · 2019Article
- Development ofFrontiers in veterinary science · 2019Article
- Antibody and cytokine response to Cystoisospora suis infections in immune-competent young pigs.Parasites & vectors · 2018Article
- Article
- Experimentally confirmed toltrazuril resistance in a field isolate of Cystoisospora suis.Parasites & vectors · 2017Article
- The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates.International journal for parasitology · 2017Article
- Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein.Parasites & vectors · 2017Article
- Vaccination against parasites - status quo and the way forward.Porcine health management · 2016Review
- Cystoisospora suis - A Model of Mammalian Cystoisosporosis.Frontiers in veterinary science · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coccidian parasites are of major importance in animal production, public health and food safety. The most frequently used representative in basic research on this group is Toxoplasma gondii. Although this parasite is well investigated there is no adequate in vitro model for its sexual development available and knowledge on this important life cycle phase is therefore scarce. The use of Isosporasuis, a sister taxon to T. gondii and the causative agent of piglet coccidiosis, could provide a solution for this. In the present study an in vitro model for neonatal porcine coccidiosis in cells representative for the in vivo situation in the piglet gut was developed and evaluated. The parasite development was investigated by light and transmission electron microscopy and optimum culture conditions were evaluated. Intestinal porcine epithelial cells (IPEC-J2) adequately representing the natural host cells supported the development of all endogenous life cycle stages of I. suis, including gametocytes and oocysts. A concentration of 5% fetal calf serum in the culture medium led to highest gametocyte densities on day 12 post infection. Low infection doses (≤1 sporozoite for 100 host cells) were best for oocyst and gametocyte development. The presented system can also be used for immunostaining with established antibodies developed against T. gondii (in our case, anti-TgIMC3 antibodies directed against the inner membrane complex 3). The complete life cycle of I. suis in a cell line representing the natural host cell type and species provides a unique model among coccidian parasites and can be used to address a wide range of topics, especially with regard to the sexual development of coccidia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.