ArticlePloS one2013
The co-expression pattern of odorant binding proteins and olfactory receptors identify distinct trichoid sensilla on the antenna of the malaria mosquito Anopheles gambiae.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Pleiotropic Function of Antenna-Specific Odorant-Binding Protein Links Xenobiotic Adaptation and Olfaction inInsects · 2025Article
- Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types.BMC biology · 2024Article
- Mutually Exclusive Expression of Closely Related Odorant-Binding Proteins 9A and 9B in the Antenna of the Red Flour BeetleBiomolecules · 2021Article
- Putative ligand binding sites of two functionally characterized bark beetle odorant receptors.BMC biology · 2021Article
- Olfaction in Anopheles mosquitoes.Chemical senses · 2021Review
- Olfactory systems across mosquito species.Cell and tissue research · 2021Review
- Expression of odorant-binding proteins in mouthpart palps of the desert locust Schistocerca gregaria.Insect molecular biology · 2019Article
- Access to the odor world: olfactory receptors and their role for signal transduction in insects.Cellular and molecular life sciences : CMLS · 2018Review
- Odorant Binding Proteins of the Desert LocustFrontiers in physiology · 2018Article
- Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization.Journal of visualized experiments : JoVE · 2017Article
- Organization and function ofeLife · 2016Article
- Organization of olfactory centres in the malaria mosquito Anopheles gambiae.Nature communications · 2016Article
- Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata.Scientific reports · 2016Article
- Morphology and Ultrastructure of Antennal Sensilla in Male and Female Agrilus mali (Coleoptera: Buprestidae).Journal of insect science (Online) · 2016Article
- Antennal RNA-sequencing analysis reveals evolutionary aspects of chemosensory proteins in the carpenter ant, Camponotus japonicus.Scientific reports · 2015Article
- Pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in Chilo suppressalis.Scientific reports · 2015Article
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- The blunt trichoid sensillum of female mosquitoes, Anopheles gambiae: odorant binding protein and receptor types.International journal of biological sciences · 2014Article
- Variant ionotropic receptors are expressed in olfactory sensory neurons of coeloconic sensilla on the antenna of the desert locust (Schistocerca gregaria).International journal of biological sciences · 2013Article
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7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial steps of odorant recognition in the insect olfactory system involve odorant binding proteins (OBPs) and odorant receptors (ORs). While large families of OBPs have been identified in the malaria vector A. gambiae, little is known about their expression pattern in the numerous sensory hairs of the female antenna. We applied whole mount fluorescence in Situ hybridization (WM-FISH) and fluorescence immunohistochemistry (WM-FIHC) to investigate the sensilla co-expression of eight A. gambiae OBPs (AgOBPs), most notably AgOBP1 and AgOBP4, which all have abundant transcripts in female antenna. WM-FISH analysis of female antennae using AgOBP-specific probes revealed marked differences in the number of cells expressing each various AgOBPs. Testing combinations of AgOBP probes in two-color WM-FISH resulted in distinct cellular labeling patterns, indicating a combinatorial expression of AgOBPs and revealing distinct AgOBP requirements for various functional sensilla types. WM-FIHC with antisera to AgOBP1 and AgOBP4 confirmed expression of the respective proteins by support cells and demonstrated a location of OBPs within sensilla trichodea. Based on the finding that AgOBP1 and AgOBP4 as well as the receptor type AgOR2 are involved in the recognition of indole, experiments were performed to explore if the AgOBP-types and AgOR2 are co-expressed in distinct olfactory sensilla. Applying two-color WM-FISH with AgOBP-specific probes and probes specific for AgOR2 revealed a close association of support cells bearing transcripts for AgOBP1 and AgOBP4 and neurons with a transcript for the receptor AgOR2. Moreover, combined WM-FISH/-FIHC approaches using an AgOR2-specific riboprobe and AgOBP-specific antisera revealed the expression of the "ligand-matched" AgOBP1, AgOBP4 and AgOR2 to single trichoid hairs. This result substantiates the notion that a specific response to indole is mediated by an interplay of the proteins.
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