ArticlePloS one2013
Transcriptome analysis in sheepgrass (Leymus chinensis): a dominant perennial grass of the Eurasian Steppe.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- A PCA-random forest pipeline for high-resolution SNP-based cultivar discrimination in Leymus chinensis.BMC plant biology · 2026Article
- An integrative multi-omics investigation into the influence of forage type on the volatile flavor profile of Ujumqin sheep mutton.Frontiers in veterinary science · 2026Article
- Effects of different additives on fermentation characteristics, nutrient composition and microbial communities of Leymus chinensis silage.BMC microbiology · 2025Article
- The Combination of Physiological and Transcriptomic Approaches Reveals New Insights into the Molecular Mechanisms ofInternational journal of molecular sciences · 2025Article
- Development of Genome-Wide SSR Markers inInternational journal of molecular sciences · 2025Article
- The effect of modifier and a water-soluble fertilizer on two forages grown in saline-alkaline soil.PloS one · 2024Article
- Phosphorus availability and planting patterns regulate soil microbial effects on plant performance in a semiarid steppe.Annals of botany · 2023Article
- Article
- Clonal dominant grassFrontiers in plant science · 2023Article
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- Transcriptome of Endophyte-Positive and Endophyte-Free Tall Fescue Under Field Stresses.Frontiers in plant science · 2022Article
- Full-length transcriptome sequencing analysis and characterization, development and validation of microsatellite markers inFrontiers in plant science · 2022Article
- Ectopic Expression of a Salt-Inducible Gene,Genes · 2021Article
- Transcriptome sequencing and gene expression profiling ofBreeding science · 2021Article
- Transcriptomic resources for prairie grass (Bromus catharticus): expressed transcripts, tissue-specific genes, and identification and validation of EST-SSR markers.BMC plant biology · 2021Article
- Rhizosphere bacteria community and functions under typical natural halophyte communities in North China salinized areas.PloS one · 2021Article
- From the Outside to the Inside: New Insights on the Main Factors That Guide Seed Dormancy and Germination.Genes · 2020Review
- The Effect of Replacing Wildrye Hay with Mulberry Leaves on the Growth Performance, Blood Metabolites, and Carcass Characteristics of Sheep.Animals : an open access journal from MDPI · 2020Article
- LcMYB4, an unknown function transcription factor gene from sheepgrass, as a positive regulator of chilling and freezing tolerance in transgenic Arabidopsis.BMC plant biology · 2020Article
- Effects of Flag Leaf and Number of Vegetative Ramets on Sexual Reproductive Performance in the Clonal GrassFrontiers in plant science · 2020Article
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSheepgrass [Leymus chinensis (Trin.) Tzvel.] is an important perennial forage grass across the Eurasian Steppe and is known for its adaptability to various environmental conditions. However, insufficient data resources in public databases for sheepgrass limited our understanding of the mechanism of environmental adaptations, gene discovery and molecular marker development.
resultsThe transcriptome of sheepgrass was sequenced using Roche 454 pyrosequencing technology. We assembled 952,328 high-quality reads into 87,214 unigenes, including 32,416 contigs and 54,798 singletons. There were 15,450 contigs over 500 bp in length. BLAST searches of our database against Swiss-Prot and NCBI non-redundant protein sequences (nr) databases resulted in the annotation of 54,584 (62.6%) of the unigenes. Gene Ontology (GO) analysis assigned 89,129 GO term annotations for 17,463 unigenes. We identified 11,675 core Poaceae-specific and 12,811 putative sheepgrass-specific unigenes by BLAST searches against all plant genome and transcriptome databases. A total of 2,979 specific freezing-responsive unigenes were found from this RNAseq dataset. We identified 3,818 EST-SSRs in 3,597 unigenes, and some SSRs contained unigenes that were also candidates for freezing-response genes. Characterizations of nucleotide repeats and dominant motifs of SSRs in sheepgrass were also performed. Similarity and phylogenetic analysis indicated that sheepgrass is closely related to barley and wheat.
conclusionsThis research has greatly enriched sheepgrass transcriptome resources. The identified stress-related genes will help us to decipher the genetic basis of the environmental and ecological adaptations of this species and will be used to improve wheat and barley crops through hybridization or genetic transformation. The EST-SSRs reported here will be a valuable resource for future gene-phenotype studies and for the molecular breeding of sheepgrass and other Poaceae species.
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