ArticleDevelopment (Cambridge, England)2013
Cell and tissue dynamics during Tribolium embryogenesis revealed by versatile fluorescence labeling approaches.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed.
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- Spatiotemporal segmentation of contraction waves in the extra-embryonic membranes of the red flour beetle.BMC bioinformatics · 2025Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
- Embryogenesis in Myrmicine Ants Combines Features of Short Germ-Band Development With a Progressive Mode of Segmentation.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- Vertex models capturing subcellular scales in epithelial tissues.PLoS computational biology · 2025Review
- The cryptonephridial/rectal complex: an evolutionary adaptation for water and ion conservation.Biological reviews of the Cambridge Philosophical Society · 2025Review
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- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- Sculpting with stiffness: rigidity as a regulator of morphogenesis.Biochemical Society transactions · 2023Review
- Nfinder: automatic inference of cell neighborhood in 2D and 3D using nuclear markers.BMC bioinformatics · 2023Article
- The extended analogy of extraembryonic development in insects and amniotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Review
- The organizing role of Wnt signaling pathway during arthropod posterior growth.Frontiers in cell and developmental biology · 2022Review
- Virtual spherical-shaped multicellular platform for simulating the morphogenetic processes of spider-like body axis formation.Frontiers in cell and developmental biology · 2022Article
- QuickPIV: Efficient 3D particle image velocimetry software applied to quantifying cellular migration during embryogenesis.BMC bioinformatics · 2021Article
- Communal living: the role of polyploidy and syncytia in tissue biology.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
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Abstract
Studies on new arthropod models such as the beetle Tribolium castaneum are shifting our knowledge of embryonic patterning and morphogenesis beyond the Drosophila paradigm. In contrast to Drosophila, Tribolium embryos exhibit the short-germ type of development and become enveloped by extensive extra-embryonic membranes, the amnion and serosa. The genetic basis of these processes has been the focus of active research. Here, we complement genetic approaches with live fluorescence imaging of Tribolium embryos to make the link between gene function and morphogenetic cell behaviors during blastoderm formation and differentiation, germband condensation and elongation, and extra-embryonic development. We first show that transient labeling methods result in strong, homogeneous and persistent expression of fluorescent markers in Tribolium embryos, labeling the chromatin, membrane, cytoskeleton or combinations thereof. We then use co-injection of fluorescent markers with dsRNA for live imaging of embryos with disrupted caudal gene function caused by RNA interference. Using these approaches, we describe and compare cell and tissue dynamics in Tribolium embryos with wild-type and altered fate maps. We find that Tribolium germband condensation is effected by cell contraction and intercalation, with the latter being dependent on the anterior-posterior patterning system. We propose that germband condensation drives initiation of amnion folding, whereas expansion of the amniotic fold and closure of the amniotic cavity are likely driven by contraction of an actomyosin cable at the boundary between the amnion and serosa. Our methodology provides a comprehensive framework for testing quantitative models of patterning, growth and morphogenetic mechanisms in Tribolium and other arthropod species.
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