ArticleWorld journal of microbiology & biotechnology2014
Investigation on the mechanisms for biotransformation of saponins to diosgenin.
Article in World journal of microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- The Isolation, Identification and Immobilization Method of Three Novel Enzymes with Diosgenin-Producing Activity Derived from anInternational journal of molecular sciences · 2023Article
- Diosgenin: An Updated Pharmacological Review and Therapeutic Perspectives.Oxidative medicine and cellular longevity · 2022Review
- Synthesis and Pharmacological Effects of Diosgenin-Betulinic Acid Conjugates.Molecules (Basel, Switzerland) · 2020Article
- Therapeutic Potential of Diosgenin and Its Major Derivatives against Neurological Diseases: Recent Advances.Oxidative medicine and cellular longevity · 2020Review
- Biotransformation of steroidal saponins in sisal (Biotechnology, biotechnological equipment · 2014Article
- Evaluation of different pretreatments on microbial transformation of saponins inBiotechnology, biotechnological equipment · 2014Article
- In situ pressurized biphase acid hydrolysis, a promising approach to produce bioactive diosgenin from the tubers of Dioscorea Zingiberensis.Pharmacognosy magazineArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to improve the efficiency of biotransformation of saponins in Dioscorea zingiberensis to diosgenin, a new enzymatic model was developed to investigate the mechanism of the metabolic systems. Four main saponin hydrolases (E1, E2, E3 and E4) were purified from Trichoderma reesei. Using progracillin as substrate, the enzymatic hydrolysis experiments with E1, E2, E3 and E4 were carried out respectively. Saponin concentrations during each biotransformation reaction were constructed with a kinetic model consisting of a few Michaelis-Menten equations. During biotransformation, C-26 glycoside and C-3 terminal glycoside were cleaved sequentially from saponins by E1, E2, E3 and E4. Then C-3 terminal rhamnoside and C-3 glycoside were released from the aglycone stepwisely by E2 and E3, to yield diosgenin. E2 and E3 were the key enzymes in the system, and cleavage of the C-3 glycoside from saponins was the rate-limiting step in the biotransformation process. The proposed enzymatic model might be used to analyze the mechanism for biotransformation of saponins to diosgenin.
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