ArticleInvestigative ophthalmology & visual science2013
Substratum compliance modulates corneal fibroblast to myofibroblast transformation.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Effect of Decorin and Aligned Collagen Fibril Topography on TGF-β1 Activation of Corneal Keratocytes.Bioengineering (Basel, Switzerland) · 2025Article
- Corneal Keratocytes, Fibroblasts, and Myofibroblasts Exhibit Distinct Transcriptional Profiles In Vitro.Investigative ophthalmology & visual science · 2025Article
- Effects of Cell Seeding Density, Extracellular Matrix Composition, and Geometry on Yes-Associated Protein Translocation in Corneal Fibroblasts.International journal of molecular sciences · 2025Article
- Squishy matters - Corneal mechanobiology in health and disease.Progress in retinal and eye research · 2024Review
- Use of Nanoindentation in Determination of Regional Biomechanical Properties of Rabbit Cornea After UVA Cross-Linking.Investigative ophthalmology & visual science · 2023Article
- The impact of UV cross-linking on corneal stromal cell migration, differentiation and patterning.Experimental eye research · 2023Article
- Unravelling the Impact of Cyclic Mechanical Stretch in Keratoconus-A Transcriptomic Profiling Study.International journal of molecular sciences · 2023Article
- The TGM2 inhibitor cysteamine hydrochloride does not impact corneal epithelial and stromal wound healing in vitro and in vivo.Experimental eye research · 2023Article
- Collagen crosslinking impacts stromal wound healing and haze formation in a rabbit phototherapeutic keratectomy model.Molecular vision · 2023Article
- In Situ-Forming Collagen-Hyaluronate Semi-Interpenetrating Network Hydrogel Enhances Corneal Defect Repair.Translational vision science & technology · 2022Article
- BMP3 inhibits TGFβ2-mediated myofibroblast differentiation during wound healing of the embryonic cornea.NPJ Regenerative medicine · 2022Article
- ECM stiffness modulates the proliferation but not the motility of primary corneal keratocytes in response to PDGF-BB.Experimental eye research · 2022Article
- Signaling Downstream of Focal Adhesions Regulates Stiffness-Dependent Differences in the TGF-Frontiers in cell and developmental biology · 2022Article
- Unraveling the mechanobiology of cornea: From bench side to the clinic.Frontiers in bioengineering and biotechnology · 2022Review
- Metal Oxide Engineered Nanomaterials Modulate Rabbit Corneal Fibroblast to Myofibroblast Transformation.Translational vision science & technology · 2021Article
- Transcorneal delivery of topically applied silver nanoparticles does not delay epithelial wound healing.NanoImpact · 2021Article
- Engineered microenvironment for the study of myofibroblast mechanobiology.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2021Article
- Differential effects of Hsp90 inhibition on corneal cells in vitro and in vivo.Experimental eye research · 2021Article
- ECM Stiffness Controls the Activation and Contractility of Corneal Keratocytes in Response to TGF-β1.Biophysical journal · 2020Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
purposeThe transformation of fibroblasts to myofibroblasts is critical to corneal wound healing, stromal haze formation, and scarring. It has recently been demonstrated that the provision of biomimetic substratum topographic cues inhibits the progression toward the myofibroblast phenotype under the influence of transforming growth factor β1 (TGF-β1). The objective of this study was to determine the effect of another fundamental biophysical cue, substrate compliance, on TGF-β1-induced myofibroblast transformation of primary corneal cells isolated from human and rabbit corneas.
methodsHuman and rabbit corneal fibroblasts were cultured on surfaces of varying substrate compliance (4-71 kPa) and tissue culture plastic (TCP) (> 1 gigapascal [GPa]). Cells were cultured in media containing TGF-β1 at concentrations of 0, 1, or 10 ng/mL for 72 hours. RNA and protein were collected from cells cultured on polyacrylamide gels and TCP and were analyzed for the expression of α-smooth muscle actin (α-SMA), a key marker of myofibroblast transformation, using quantitative PCR, immunocytochemistry, and Western blot.
resultsCells grown on more compliant substrates demonstrated significantly reduced amounts of α-SMA mRNA compared with TCP. Immunocytochemistry and Western blot analysis determining the presence of α-SMA corroborated this finding, thus confirming a reduced transformation to the myofibroblast phenotype on more compliant substrates compared with cells on TCP in the presence of TGF-β1.
conclusionsThese data indicate that substrate compliance modulates TGF-β1-induced expression of α-SMA and thus influences myofibroblast transformation in the corneal stroma. This provides further evidence that biomimetic biophysical cues inhibit myofibroblast transformation and participate in stabilizing the native cellular phenotype.
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