ArticleIn vitro cellular & developmental biology. Animal2013
Characterization of primary human skeletal muscle cells from multiple commercial sources.
Article in In vitro cellular & developmental biology. Animal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines.Molecular metabolism · 2017Trial
- Aligned basement membrane-modified collagen scaffolds for skeletal muscle tissue engineering.bioRxiv : the preprint server for biology · 2026Article
- Ultrasound-guided skeletal muscle biopsy technique permits measurement of structural, functional, cellular and biochemical properties.Scientific reports · 2026Article
- Recombinant ADAMTS‑1 promotes muscle regeneration accompanied by downregulation of Notch signaling.International journal of molecular medicine · 2026Article
- Insights into human muscle biology from human primary skeletal muscle cell culture.Journal of muscle research and cell motility · 2025Review
- Advanced High-Content Phenotypic Screening to Identify Drugs That Ameliorate the Inhibition of Skeletal Muscle Cell Differentiation Induced by Cancer Cachexia Serum.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Actuating Extracellular Matrices Decouple the Mechanical and Biochemical Effects of Muscle Contraction on Motor Neurons.Advanced healthcare materials · 2025Article
- Hypotonic Swelling Method for the Isolation of Pure Mitochondria From Primary Human Skeletal Myoblasts for Proteomic Studies.Journal of cellular and molecular medicine · 2025Article
- Identifying the potential therapeutic effects of miR‑6516 on muscle disuse atrophy.Molecular medicine reports · 2024Article
- Electron microscopic analysis of the influence of iPSC-derived motor neurons on bioengineered human skeletal muscle tissues.Cell and tissue research · 2024Article
- Zebrafish Models for Skeletal Muscle Senescence: Lessons from Cell Cultures and Rodent Models.Molecules (Basel, Switzerland) · 2022Review
- Comparative oncology reveals DNMT3B as a molecular vulnerability in undifferentiated pleomorphic sarcoma.Cellular oncology (Dordrecht, Netherlands) · 2022Article
- Effect of Tomato Peel Extract Grown under Drought Stress Condition in a Sarcopenia Model.Molecules (Basel, Switzerland) · 2022Article
- Functional impact and targetability ofCold Spring Harbor molecular case studies · 2022Article
- Free Fatty Acid Species Differentially Modulate the Inflammatory Gene Response in Primary Human Skeletal Myoblasts.Biology · 2021Article
- Natural Extracts to Augment Energy Expenditure as a Complementary Approach to Tackle Obesity and Associated Metabolic Alterations.Biomolecules · 2021Article
- Tempora: Cell trajectory inference using time-series single-cell RNA sequencing data.PLoS computational biology · 2020Article
- Duchenne muscular dystrophy hiPSC-derived myoblast drug screen identifies compounds that ameliorate disease in mdx mice.JCI insight · 2020Article
- Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds.ACS biomaterials science & engineering · 2020Article
- Asymmetric cellular responses in primary human myoblasts using sera of different origin and specification.PloS one · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a significant unmet need for safe, anabolic muscle therapies to treat diseases and conditions associated with severe muscle weakness and frailty. The identification of such therapies requires appropriate cell-based screening assays to select compounds for further development using animal models. Primary human skeletal muscle cells have recently become available from a number of commercial vendors. Such cells may be valuable for studying the mechanisms that direct muscle differentiation, and for identifying and characterizing novel therapeutic approaches for the treatment of age- and injury-induced muscle disorders. However, only limited characterization of these cells has been reported to date. Therefore, we have examined four primary human muscle cell preparations from three different vendors for their capacity to differentiate into multinucleated myotubes. Two of the preparations demonstrated robust myotube formation and expressed characteristic markers of muscle differentiation. Furthermore, these myotubes could be induced to undergo morphological atrophy- and hypertrophy-like responses, and atrophy could be blocked with an inhibitor of myostatin signaling, a pathway that is known to negatively regulate muscle mass. Finally, the myotubes were efficiently infected with recombinant adenovirus, providing a tool for genetic modification. Taken together, our results indicate that primary human muscle cells can be a useful system for studying muscle differentiation, and may also provide tools for studying new therapeutic molecules for the treatment of muscle disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.