ArticleEuropean journal of human genetics : EJHG2014
An efficient method for long-term room temperature storage of RNA.
Article in European journal of human genetics : EJHG, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 147 citations in OpenAlex.
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- The mass spectrometric intact transition epitope mapping method supports protein engineering of foldon trimer variants.Scientific reports · 2025Article
- Long-Term Stabilization of Dengue Virus RNA at 37 °C for 14 Months Using Silk Fibroin Films.Viruses · 2025Article
- Viral persistence in long COVID: Research advances and treatment strategies.Infectious diseases & immunity · 2025Review
- Exploring potential biomarkers in foods of animal origin.Journal of food science and technology · 2025Review
- Technical Considerations of Pharmacokinetic Assays for LNP-mRNA Drug Products by RT-qPCR.The AAPS journal · 2025Article
- Analytical approach for identification and mechanistic insights into mRNA-lipid adduct formation.Molecular therapy. Nucleic acids · 2025Article
- Self-splicing RNA circularization facilitated by intact group I and II introns.Nature communications · 2025Article
- Retrospective analysis and decentralized distribution to improve the lifecycle of Ah receptor research assets.Biochemical pharmacology · 2025Article
- Aptamer-Functionalized Platform for Selective Bacterial Isolation and Rapid RNA Purification Using Capture Pins.Sensors (Basel, Switzerland) · 2025Article
- Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification.Open life sciences · 2025Article
- Analysis of metagenomic data.Nature reviews. Methods primers · 2025Article
- The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis.International journal of molecular sciences · 2024Article
- RNA Stability: A Review of the Role of Structural Features and Environmental Conditions.Molecules (Basel, Switzerland) · 2024Review
- Review
- Molecular Dynamics Simulation of Lipid Nanoparticles Encapsulating mRNA.Molecules (Basel, Switzerland) · 2024Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA is a tool used in many fields, from molecular and cellular biology to medicine and nanotechnology. For most of these uses, the integrity of RNA is required and must be maintained during storage. Even though freezing is currently the storage method of choice, the increasing number of samples to be stored and the costly use of a cold chain have highlighted the need for room temperature preservation methods. Here, we report a new room temperature technology that consists in drying RNA samples in the presence of a stabilizer in stainless steel minicapsules. These air- and water-tight capsules isolate RNA from the atmosphere and maintain an anhydrous and anoxic environment. Through the evaluation of RNA integrity over time at room temperature or 90 °C, we identified atmospheric humidity as a major deleterious factor. The degradation rate dependence in temperature fitted an Arrhenius model, with an activation energy of 28.5 kcal/mol and an extrapolated room temperature degradation rate of 3.2 10(-13)/nt/s (95% confidence interval: 2.3-4.2/nt/s). In these conditions, it is expected that an RNA molecule will be subjected to 0.7-1.3 cut every 1000 nucleotides per century. In addition, we showed that stored RNA is compatible for further analyses, such as reverse transcription-quantitative PCR. No significant change in the Cq values was observed over a simulated period of several decades. At last, our data are consistent with a sequence-independent degradation rate of RNA in the solid state.
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Registered trials
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