ArticleProceedings of the National Academy of Sciences of the United States of America2013
Characterization of the nitric oxide reductase from Thermus thermophilus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Metabolic division of labor drives estuarine-coastal N2O emissions.The ISME journal · 2026Article
- Insertion of the FeFEBS letters · 2025Article
- Diversity and evolution of nitric oxide reduction in bacteria and archaea.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bacterial denitrifying nitric oxide reductases and aerobic respiratory terminal oxidases use similar delivery pathways for their molecular substrates.Biochimica et biophysica acta. Bioenergetics · 2018Article
- Nitric Oxide Accumulation: The Evolutionary Trigger for Phytopathogenesis.Frontiers in microbiology · 2017Article
- Article
- The evolution of respiratory O2/NO reductases: an out-of-the-phylogenetic-box perspective.Journal of the Royal Society, Interface · 2014Review
- A third subunit in ancestral cytochrome c-dependent nitric oxide reductases.Applied and environmental microbiology · 2014Article
- Transferable denitrification capability of Thermus thermophilus.Applied and environmental microbiology · 2014Review
- The nitric-oxide reductase from Paracoccus denitrificans uses a single specific proton pathway.The Journal of biological chemistry · 2013Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Nitrous oxide (N2O) is a powerful greenhouse gas implicated in climate change. The dominant source of atmospheric N2O is incomplete biological dentrification, and the enzymes responsible for the release of N2O are NO reductases. It was recently reported that ambient emissions of N2O from the Great Boiling Spring in the United States Great Basin are high, and attributed to incomplete denitrification by Thermus thermophilus and related bacterial species [Hedlund BP, et al. (2011) Geobiology 9(6)471-480]. In the present work, we have isolated and characterized the NO reductase (NOR) from T. thermophilus. The enzyme is a member of the cNOR family of enzymes and belongs to a phylogenetic clade that is distinct from previously examined cNORs. Like other characterized cNORs, the T. thermophilus cNOR consists of two subunits, NorB and NorC, and contains a one heme c, one Ca(2+), a low-spin heme b, and an active site consisting of a high-spin heme b and FeB. The roles of conserved residues within the cNOR family were investigated by site-directed mutagenesis. The most important and unexpected result is that the glutamic acid ligand to FeB is not essential for function. The E211A mutant retains 68% of wild-type activity. Mutagenesis data and the pattern of conserved residues suggest that there is probably not a single pathway for proton delivery from the periplasm to the active site that is shared by all cNORs, and that there may be multiple pathways within the T. thermophilus cNOR.
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