ArticleMolecular and cellular biology2013
Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Efficient, complete biosynthesis of isoflavonoid calycosin-7-glucoside by metabolically engineered yeast cell factories.Frontiers in bioengineering and biotechnology · 2026Article
- Transcription factor clusters enable target search but do not contribute to target gene activation.Nucleic acids research · 2023Article
- De novo biosynthesis of bioactive isoflavonoids by engineered yeast cell factories.Nature communications · 2021Article
- Decoupling transcription factor expression and activity enables dimmer switch gene regulation.Science (New York, N.Y.) · 2021Article
- Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering.ACS synthetic biology · 2020Article
- Monitoring of switches in heterochromatin-induced silencing shows incomplete establishment and developmental instabilities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Modeling transcriptional activation changes to Gal4 variants via structure-based computational mutagenesis.PeerJ · 2018Article
- Genetic and Epigenetic Strategies Potentiate Gal4 Activation to Enhance Fitness in Recently Diverged Yeast Species.Current biology : CB · 2017Article
- Polymorphisms in the yeast galactose sensor underlie a natural continuum of nutrient-decision phenotypes.PLoS genetics · 2017Article
- Regulating the UAS/GAL4 system in adultPeerJ · 2017Article
- Ongoing resolution of duplicate gene functions shapes the diversification of a metabolic network.eLife · 2016Article
- Analyzing negative feedback using a synthetic gene network expressed in the Drosophila melanogaster embryo.BMC systems biology · 2016Article
- The yeast galactose network as a quantitative model for cellular memory.Molecular bioSystems · 2015Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The DNA-binding transcriptional activator Gal4 and its regulators Gal80 and Gal3 constitute a galactose-responsive switch for the GAL genes of Saccharomyces cerevisiae. Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain (AD). In the absence of galactose, a Gal80 dimer binds to a dimer of Gal4, masking the Gal4AD. Galactose triggers Gal3-Gal80 interaction to rapidly initiate Gal4-mediated transcription activation. Just how Gal3 alters Gal80 to relieve Gal80 inhibition of Gal4 has been unknown, but previous analyses of Gal80 mutants suggested a possible competition between Gal3-Gal80 and Gal80 self-association interactions. Here we assayed Gal80-Gal80 interactions and tested for effects of Gal3. Immunoprecipitation, cross-linking, and denaturing and native PAGE analyses of Gal80 in vitro and fluorescence imaging of Gal80 in live cells show that Gal3-Gal80 interaction occurs concomitantly with a decrease in Gal80 multimers. Consistent with this, we find that newly discovered nuclear clusters of Gal80 dissipate in response to galactose-triggered Gal3-Gal80 interaction. We discuss the effect of Gal3 on the quaternary structure of Gal80 in light of the evidence pointing to multimeric Gal80 as the form required to inhibit Gal4.
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