ArticleThe Journal of biological chemistry2013
Three-dimensional structure of the toxin-delivery particle antifeeding prophage of Serratia entomophila.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 64 citations in OpenAlex.
- Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes.Nature biotechnology · 2026Article
- Stepwise firing mechanism of an extracellular contractile injection system.Nature communications · 2026Article
- Structure of a contractile injection system in Salmonella enterica subsp. salamae.Nature communications · 2026Article
- The landscape of bacterial contractile injection systems across large-scale metagenomes.Microbiology spectrum · 2025Article
- Molecular mechanism of bacteriophage contraction structure of an S-layer-penetrating bacteriophage.Life science alliance · 2025Article
- Tradeoffs and constraints on the evolution of tailocins.Trends in microbiology · 2024Review
- Identification of novel toxins associated with the extracellular contractile injection system using machine learning.Molecular systems biology · 2024Article
- Tyrosine-modifying glycosylation by Yersinia effectors.The Journal of biological chemistry · 2024Article
- Article
- A contractile injection system is required for developmentally regulated cell death in Streptomyces coelicolor.Nature communications · 2023Article
- Evolutionary and ecological role of extracellular contractile injection systems: from threat to weapon.Frontiers in microbiology · 2023Review
- High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers.Nature communications · 2022Article
- Biological Functions and Applications of Virus-Related Bacterial Nanoparticles: A Review.International journal of molecular sciences · 2022Review
- Investigating the Process of Sheath Maturation in Antifeeding Prophage: a Phage Tail-Like Protein Translocation Structure.Journal of bacteriology · 2021Article
- The extracellular contractile injection system is enriched in environmental microbes and associates with numerous toxins.Nature communications · 2021Article
- A Distinct Contractile Injection System Gene Cluster Found in a Majority of Healthy Adult Human Microbiomes.mSystems · 2020Article
- Double Tubular Contractile Structure of the Type VI Secretion System Displays Striking Flexibility and Elasticity.Journal of bacteriology · 2019Article
- Atomic structures of an entire contractile injection system in both the extended and contracted states.Nature microbiology · 2019Article
- TheeLife · 2019Article
- A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector.eLife · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The Serratia entomophila antifeeding prophage (Afp) is a bullet-shaped toxin-delivery apparatus similar to the R-pyocins of Pseudomonas aeruginosa. Morphologically it resembles the sheathed tail of bacteriophages such as T4, including a baseplate at one end. It also shares features with the type VI secretion systems. Cryo-electron micrographs of tilted Afp specimens (up to 60 degrees) were analyzed to determine the correct cyclic symmetry to overcome the limitation imposed by exclusively side views in nominally untilted specimens. An asymmetric reconstruction shows clear 6-fold cyclic symmetry contrary to a previous conclusion of 4-fold symmetry based on analysis of only the preferred side views (Sen, A., Rybakova, D., Hurst, M. R., and Mitra, A. K. (2010) J. Bacteriol. 192, 4522-4525). Electron tomography of negatively stained Afp revealed right-handed helical striations in many of the particles, establishing the correct hand. Higher quality micrographs of untilted specimens were processed to produce a reconstruction at 2.0-nm resolution with imposed 6-fold symmetry. The helical parameters of the sheath were determined to be 8.14 nm for the subunit rise along and 40.5° for the rotation angle around the helix. The sheath is similar to that of the T4 phage tail but with a different arrangement of the subdomain of the polymerizing sheath protein(s). The central tube is similar to the diameter and axial width of the Hcp1 hexamer of P. aeruginosa type VI secretion system. The tube extends through the baseplate into a needle resembling the "puncture device" of the T4 tail. The tube contains density that may be the toxin and/or a length-determining protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.