ArticleAMB Express2013
Optimization of macroelement concentrations, pH and osmolarity for triacylglycerol accumulation in Rhodococcus opacus strain PD630.
Article in AMB Express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 24 citations in OpenAlex.
- Recent advances in microbial production of odd-chain fatty acids.World journal of microbiology & biotechnology · 2026Review
- Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals.Scientific reports · 2024Article
- Using 1-propanol to significantly enhance the production of valuable odd-chain fatty acids by Rhodococcus opacus PD630.World journal of microbiology & biotechnology · 2019Article
- Bacterial lipid droplets bind to DNA via an intermediary protein that enhances survival under stress.Nature communications · 2017Article
- Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels.Biotechnology for biofuels · 2014Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The refinement of biodiesel or renewable diesel from bacterial lipids has a great potential to make a contribution for energy production in the future. This study provides new data concerning suitable nutrient concentrations for cultivation of the Gram-positive Rhodococcus opacus PD630, which is able to accumulate large amounts of lipids during nitrogen limitation. Enhanced concentrations of magnesium have been shown to increase the final optical density and the lipid content of the cells. Elevated phosphate concentrations slowed down the onset of the accumulation phase, without a clear effect on the final optical density and the cell's lipid content. A robust growth of R. opacus was possible in the presence of ammonium concentrations of up to 1.4 g l(-1) and sucrose concentrations of up to 240 g l(-1), with an optimum regarding growth and lipid storage observed in the range of 0.2 to 0.4 g l(-1) ammonium and 20 to 40 g l(-1) sucrose, respectively. Moreover, R. opacus showed tolerance to high salt concentrations.
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Registered trials
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