ArticleProceedings of the National Academy of Sciences of the United States of America1990
Physical mapping of human chromosomes by repetitive sequence fingerprinting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Probing mercury(II)-DNA interactions by nanopore stochastic sensing.The journal of physical chemistry. B · 2013Article
- Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Non-B DNA structure-induced genetic instability and evolution.Cellular and molecular life sciences : CMLS · 2010Review
- ODS2: a multiplatform software application for creating integrated physical and genetic maps.Genetics · 2001Article
- Contigs built with fingerprints, markers, and FPC V4.7.Genome research · 2000Article
- High-resolution transcript map of the region spanning D12S1629 and D12S312 at chromosome 12q13: triple A syndrome-linked region.Genome research · 2000Article
- High throughput fingerprint analysis of large-insert clones.Genome research · 1997Article
- Random-breakage mapping method applied to human DNA sequences.Nucleic acids research · 1996Article
- Structure of the human CRFB4 gene: comparison with its IFNAR neighbor.Journal of molecular evolution · 1995Article
- Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Chromosome 16 microdeletion in a patient with juvenile neuronal ceroid lipofuscinosis (Batten disease).American journal of human genetics · 1995Article
- A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
- Evidence of linkage disequilibrium in the Spanish polycystic kidney disease I population.American journal of human genetics · 1994Article
- Detection of cryptic chromosomal abnormalities in unexplained mental retardation: a general strategy using hypervariable subtelomeric DNA polymorphisms.American journal of human genetics · 1993Review
- The human genome project.Proceedings of the National Academy of Sciences of the United States of America · 1993Review
- Low-frequency chimeric yeast artificial chromosome libraries from flow-sorted human chromosomes 16 and 21.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Genome mapping by nonrandom anchoring: a discrete theoretical analysis.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Genome organization of repetitive elements in the rodent, Peromyscus leucopus.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
- Definition, distribution, and use of a conserved Bovidae retroposon element sequence motif.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an approach for identifying overlapping cosmid clones by exploiting the high density of repetitive sequences in complex genomes. Individual clones are fingerprinted, using a combination of restriction enzyme digestions followed by hybridization with selected classes of repetitive sequences. This "repeat fingerprinting" technique allows small regions of clone overlap (10-20%) to be unambiguously assigned. We demonstrate the utility of this approach, using the fingerprinting of 3145 cosmid clones (1.25 x coverage), containing one or more (GT)n repeats, from human chromosome 16. A statistical analysis was used to link these clones into 460 contiguous sequences (contigs), averaging 106 kilobases (kb) in length and representing approximately 54% (48.7 Mb) of the euchromatic arms of this chromosome. These values are consistent with theoretical calculations and indicate that 150- to 200-kb contigs can be generated with 1.5 x coverage. This strategy requires the fingerprinting of approximately one-fourth as many cosmids as random strategies requiring 50% minimum overlap for overlap detection. By "nucleating" at specific regions in the human genome, and exploiting the high density of interspersed sequences, this approach allows (i) the rapid generation of large (greater than 100-kb) contigs in the early stages of contig mapping and (ii) the production of a contig map with useful landmarks for rapid integration of the genetic and physical maps.
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