ArticleJournal of global infectious diseases2013
Detection of invasive aspergillosis in bone marrow transplant recipients using real-time PCR.
Article in Journal of global infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- PCR in diagnosis of invasive aspergillosis: a meta-analysis of diagnostic performance.Journal of clinical microbiology · 2014Pooled it
- Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites.Current medical mycology · 2021Article
- Fatal native aortic valve fungal endocarditis caused byIDCases · 2021Article
- Fatal Prosthetic Valve Endocarditis Due toInfection and drug resistance · 2020Article
- Pathogenic Fungal Species Associated with Digestive System ofJournal of arthropod-borne diseases · 2018Article
- Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis.AMB Express · 2016Article
- A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient.Current medical mycology · 2015Article
- Co-infection of invasive pulmonary aspergillosis and cutaneous Fusarium infection in a patient with pyoderma gangrenosum.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2015Article
- Aspergillus galactomannan detection in comparison to a real-time PCR assay in serum samples from a high-risk group of patients.Central-European journal of immunology · 2015Article
- Quantification and optimization of Candida albicans DNA in blood samples using Real- Time PCR.Reports of biochemistry & molecular biology · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe invasive aspergillosis (IA) is a serious opportunistic infection caused by various species of Aspergillus in immunocompromised individuals. Basically, rapid and early diagnosis prevents IA progression. In this study we performed a Real Time PCR/ Fluorescence Resonance Energy Transfer (FRET) for diagnosis of IA in hematologic malignancies and bone marrow transplant recipients. MATERIALS AND
methodsSixty two patients with hematologic malignancies and marrow transplant recipients were evaluated for IA in Sari and Tehran from 2009 to 2010. The primer and hybridization probe were designed to amplify the specific sequence of 18S rRNA genes using Light Cycler system and FRET. Galactomannan (GM) assay was performed on serums which obtained from selected patients using the Platelia Aspergillus kit.
resultsAccording to the criteria defined by the European Organization for Research and Treatment of Cancer and Mycoses Study Group (EORTC/MSG) for IA, 18 (29%) patients out of 62 patients were stratified into probable and possible groups. The female-to-male ratio was 1:2; the mean age of the patients was 36 years. The most common malignancies in these patients were acute lymphoblastic leukemia (38.9%). The minimum detection limit was 10 conidia (10(1) CFU/ml) equivalents (100 fg) per PCR reaction. GM assay was positive in 20.9% and real-time PCR probe set assay were positive in 17.7% patients who had clinical signs and host factor according to the mentioned criteria.
conclusionUsing the Real-Time PCR/FRET assay in whole blood specimens seems to be a promising method for diagnosis of IA, especially when used in combination with the GM detection test.
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