Evidence map›Paper›PMID 23853081›Full record

ReviewCurrent protocols in human genetics2013

Gene delivery to the airway.

Nicholas W Keiser, John F Engelhardt

Abstract readReview
In one paragraph

Review in Current protocols in human genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Rapid Expansion of Human Epithelial Stem Cells Suitable for Airway Tissue Engineering.American journal of respiratory and critical care medicine · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Nicholas W KeiserUniversity of Iowa, Iowa City, Iowa, USA.
John F Engelhardt

Funding

Pulmonary Toxicology Facility CoreP30ES005605 · NIEHS · UNIVERSITY OF IOWA · PI Brandi Janssen · 1990 to 2026
$40.5M
Vector Core-Core 2P30DK054759 · NIDDK · UNIVERSITY OF IOWA · PI Alejandro Antonio Pezzulo · 1998 to 2026
$30.5M
Biology of submucosal gland stem cells in the airwayR37DK047967 · NIDDK · UNIVERSITY OF IOWA · PI ENGELHARDT, JOHN F · 2005 to 2014
$3.8M
MODELS OF SUBMUCOSAL GLAND DEVELOPMENT AND FUNCTIONR01DK047967 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI ENGELHARDT, JOHN F · 1993 to 2020
$3.8M
rAAV-directed gene therapy to the lung in cystic fibrosis ferret modelsR01HL108902 · NHLBI · UNIVERSITY OF IOWA · PI ENGELHARDT, JOHN F · 2011 to 2014
$2.2M
NHLBI NIH HHS HL108902NHLBI NIH HHS R01 HL108902NIDDK NIH HHS DK047967NIDDK NIH HHS DK54759NIDDK NIH HHS P30 DK054759NIDDK NIH HHS R01 DK047967NIDDK NIH HHS R37 DK047967NIEHS NIH HHS P30 ES005605
6 · The paper itself

Abstract

This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts, as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.

Indexed as

Gene Transfer TechniquesTransduction, GeneticAnimalsCell Culture TechniquesCell Line, TransformedEpithelial CellsGene ExpressionHeterograftsHumansModels, AnimalRespiratory MucosaTransgenes

Identifiers

PMID23853081
PMCPMC3777227

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.