ArticleJournal of bacteriology2013
Evidence for a key role of cytochrome bo3 oxidase in respiratory energy metabolism of Gluconobacter oxydans.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Valorization of Cheese Whey Powder by Two-Step Fermentation for Gluconic Acid and Ethanol Preparation.Applied biochemistry and biotechnology · 2024Article
- The respiratory chain ofFrontiers in microbiology · 2024Article
- Comparative Genomics of Acetic Acid Bacteria within the GenusMicroorganisms · 2022Article
- Oxidative Fermentation of Acetic Acid Bacteria and Its Products.Frontiers in microbiology · 2022Review
- The 5-Ketofructose Reductase ofJournal of bacteriology · 2021Article
- FNR-Type Regulator GoxR of the Obligatorily Aerobic Acetic Acid BacteriumApplied and environmental microbiology · 2021Article
- On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases.Applied microbiology and biotechnology · 2021Review
- Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose.Microbial cell factories · 2020Article
- The aerobic respiratory chain of Pseudomonas aeruginosa cultured in artificial urine media: Role of NQR and terminal oxidases.PloS one · 2020Article
- Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case ofFrontiers in bioengineering and biotechnology · 2019Review
- Oxygen Reductases in Alphaproteobacterial Genomes: Physiological Evolution From Low to High Oxygen Environments.Frontiers in microbiology · 2019Article
- Global mRNA decay and 23S rRNA fragmentation in Gluconobacter oxydans 621H.BMC genomics · 2018Article
- RNAseq analysis of α-proteobacterium Gluconobacter oxydans 621H.BMC genomics · 2018Article
- Complete Genome Sequencing and Comparative Genomic Analysis of the Thermotolerant Acetic Acid Bacterium, Acetobacter pasteurianus SKU1108, Provide a New Insight into Thermotolerance.Microbes and environments · 2016Article
- Combinatorial metabolic engineering of industrial Gluconobacter oxydans DSM2343 for boosting 5-keto-D-gluconic acid accumulation.BMC biotechnology · 2016Article
- Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticles.Microbial cell factories · 2015Article
- Utilization of D-Lactate as an Energy Source Supports the Growth of Gluconobacter oxydans.Applied and environmental microbiology · 2015Article
- Quantifying the sensitivity of G. oxydans ATCC 621H and DSM 3504 to osmotic stress triggered by soluble buffers.Journal of industrial microbiology & biotechnology · 2015Article
- A quinol oxidase, encoded by cyoABCD, is utilized to adapt to lower O2 concentrations in Rhizobium etli CFN42.Microbiology (Reading, England) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The obligatory aerobic acetic acid bacterium Gluconobacter oxydans oxidizes a variety of substrates in the periplasm by membrane-bound dehydrogenases, which transfer the reducing equivalents to ubiquinone. Two quinol oxidases, cytochrome bo3 and cytochrome bd, then catalyze transfer of the electrons from ubiquinol to molecular oxygen. In this study, mutants lacking either of these terminal oxidases were characterized. Deletion of the cydAB genes for cytochrome bd had no obvious influence on growth, whereas the lack of the cyoBACD genes for cytochrome bo3 severely reduced the growth rate and the cell yield. Using a respiration activity monitoring system and adjusting different levels of oxygen availability, hints of a low-oxygen affinity of cytochrome bd oxidase were obtained, which were supported by measurements of oxygen consumption in a respirometer. The H(+)/O ratio of the ΔcyoBACD mutant with mannitol as the substrate was 0.56 ± 0.11 and more than 50% lower than that of the reference strain (1.26 ± 0.06) and the ΔcydAB mutant (1.31 ± 0.16), indicating that cytochrome bo3 oxidase is the main component for proton extrusion via the respiratory chain. Plasmid-based overexpression of cyoBACD led to increased growth rates and growth yields, both in the wild type and the ΔcyoBACD mutant, suggesting that cytochrome bo3 might be a rate-limiting factor of the respiratory chain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.