ArticleJournal of bacteriology2013
Acetylation of the response regulator RcsB controls transcription from a small RNA promoter.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Untangling the Complexity of Two-Component Signal Transduction in Bacteria.Microorganisms · 2025Review
- The dual functions of the GTPase BipA in ribosome assembly and surface structure biogenesis in Salmonella enterica serovar Typhimurium.PLoS pathogens · 2025Article
- Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism.Nature communications · 2024Article
- RegulonDB v12.0: a comprehensive resource of transcriptional regulation in E. coli K-12.Nucleic acids research · 2024Article
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- Acetylation of Cyclic AMP Receptor Protein by Acetyl Phosphate Modulates Mycobacterial Virulence.Microbiology spectrum · 2023Article
- Sensory Systems and Transcriptional Regulation inFrontiers in bioengineering and biotechnology · 2022Review
- Acetylation of glucosyltransferases regulates Streptococcus mutans biofilm formation and virulence.PLoS pathogens · 2021Article
- Protein Acetyltransferases Mediate Bacterial Adaptation to a Diverse Environment.Journal of bacteriology · 2021Review
- Contribution of NmSystems · 2021Article
- Article
- HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar Typhimurium.BMC microbiology · 2021Article
- Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.Molecular plant pathology · 2021Article
- Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.Frontiers in microbiology · 2021Review
- Bacterial Sirtuins Overview: An Open Niche to Explore.Frontiers in microbiology · 2021Review
- The Rcs System inFrontiers in microbiology · 2021Review
- Regulation of gene expression by protein lysine acetylation in Salmonella.Journal of microbiology (Seoul, Korea) · 2020Review
- Article
- The phosphorelay BarA/SirA activates the non-cognate regulator RcsB in Salmonella enterica.PLoS genetics · 2020Article
- Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme.Nucleic acids research · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
Abstract
Nε-lysine acetylation was recently discovered on many bacterial proteins that function in diverse cellular processes. Thus, many questions remain unanswered. For example, what mechanisms regulate lysine acetylation? Does acetylation affect physiology? To help answer these questions, we studied the Escherichia coli response regulator and transcription factor RcsB, which is reported to be acetylated in vitro. To characterize RcsB acetylation, we monitored transcription from the rprA promoter, which requires RcsB. The conventional view is that RcsB is activated by phosphorylation through either the Rcs phosphorelay or acetyl phosphate. We affirmed that rprA transcription requires phosphorylated RcsB and showed that acetyl-phosphate (AcP) is a phosphoryl group donor to RcsB. However, a mutant that accumulates AcP (ackA) exhibited a reduction in rprA transcription instead of the predicted increase. rprA transcription also diminished in the cobB mutant, which lacks the only known E. coli protein deacetylase. This suggests the existence of an inhibitory mechanism that involves lysine acetylation, a supposition supported by the observation that RcsB isolated from the ackA or cobB mutant was hyperacetylated. Finally, we used a genetic approach to identify an AckA- and CobB-sensitive lysine (Lys-154) that controls RcsB activity. We propose that acetylation inhibits RcsB activity and that some of this inhibition acts through the acetylation of Lys-154.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.