ArticleJournal of clinical laboratory analysis2013
Determination of storage time of saliva samples obtained from patients with and without chronic periodontitis for the comparison of some biochemical and cytological parameters.
Article in Journal of clinical laboratory analysis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- The assessment of glutathione, glutathione peroxidase, glutathione reductase, and oxidized glutathione in patients with periodontitis-A systematic review and meta-analysis.Clinical and experimental dental research · 2024Pooled it
- Interaction between methylated arginine metabolites and oxidative stress in periodontitis: a cross-sectional study.BMC oral health · 2025Observational
- High Salivary Malondialdehyde Levels Are Associated with Periodontitis Independently of Other Risk Factors.Journal of clinical medicine · 2025Article
- Oxidative Stress and FOXO-1 Relationship in Stage III Periodontitis.Clinical oral investigations · 2024Article
- Assessment of Oxidative Stress by the Estimation of Lipid Peroxidation Marker Malondialdehyde (MDA) in Patients with Chronic Periodontitis: A Systematic Review and Meta-Analysis.International journal of dentistry · 2023Review
- Evaluation of Saliva Stability for NMR Metabolomics: Collection and Handling Protocols.Metabolites · 2020Article
- Effect of Freezing Time on Tissue Factor Activity and Macronutrients of Human Milk.The protein journal · 2020Article
- Effect of Storage Time and Temperature on Salivary Total Antioxidant Capacity, Total Oxidant Status, and Oxidant Stress Index.Acta stomatologica Croatica · 2019Article
- The antioxidant master glutathione and periodontal health.Dental research journalReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalivary glutathione (GSH), malondialdehyde (MDA), protein, sialic acid (SA) levels, cytological parameters, and tissue factor activities (TFa) were investigated when fresh and after 3, 7, 11, 15, 21, and 30 days (d) of storage at -20°C both in the control and the periodontitis group. Moreover, the control and the periodontits groups were compared and continuity of the significances detected between the two groups were evaluated.
methodsGSH, MDA, SA, protein, and TFa were determined using the methods of Beutler, Yagi, Warren, Lowry, and Quick, respectively. Saliva imprint samples were stained with Giemsa and microscopically examined.
resultsWhen the continuity of the significances of differences between the two groups was investigated, differences continued to be significant for GSH and TFa on days 3, 7, 11, 15, 21, and 30. Cytologically, only the significance detected between leucocyte numbers continued to be significant for 30 d. However significance of differences in total protein, MDA, and SA levels on day 0, were interrupted on days 3, 7, and 11, respectively.
conclusionSaliva samples may be stored for 30 d for GSH and TFa analyses in patients with and without periodontitis. However, to compare salivary MDA, SA, and total protein levels in these groups we suggest fresh samples to be studied.
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