ArticleProceedings of the National Academy of Sciences of the United States of America2013
A molt timer is involved in the metamorphic molt in Manduca sexta larvae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Molting in Pancrustacea Is Characterized by Both Deeply Conserved and Recently Evolved Gene Modules.Genome biology and evolution · 2026Article
- Lipases are differentially regulated by hormones to maintain free fatty acid homeostasis for insect brain development.BMC biology · 2024Article
- A dynamical model of growth and maturation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Settlement cue selectivity by larvae of the destructive crown-of-thorns starfish.Biology letters · 2023Article
- Influence of photoperiod on thermal responses in body size, growth and development inCurrent research in insect science · 2022Article
- Evolution of reduced minimum critical size as a response to selection for rapid pre-adult development inRoyal Society open science · 2020Article
- Review
- Divergent mechanisms for regulating growth and development after imaginal disc damage in the tobacco hornworm,The Journal of experimental biology · 2019Article
- The role of nuclear receptor E75 in regulating the molt cycle of Daphnia magna and consequences of its disruption.PloS one · 2019Article
- Metamorphosis is induced by food absence rather than a critical weight in the solitary bee,Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Nutrient-Dependent Endocycling in Steroidogenic Tissue Dictates Timing of Metamorphosis in Drosophila melanogaster.PLoS genetics · 2017Article
- Insights into How Longicorn Beetle Larvae Determine the Timing of Metamorphosis: Starvation-Induced Mechanism Revisited.PloS one · 2016Article
- A size threshold governs Caenorhabditis elegans developmental progression.Proceedings. Biological sciences · 2015Article
- Big or fast: two strategies in the developmental control of body size.BMC biology · 2015Article
- The role of juvenile hormone and insulin/TOR signaling in the growth of Manduca sexta.BMC biology · 2015Article
- Functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis.The Journal of biological chemistry · 2014Article
- Androgen receptor DNA methylation regulates the timing and androgen sensitivity of mouse prostate ductal development.Developmental biology · 2014Article
- Developmental constraints in cave beetles.Biology letters · 2014Article
- Juvenile hormone regulates body size and perturbs insulin signaling in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Manduca sexta larvae are a model for growth control in insects, particularly for the demonstration of critical weight, a threshold weight that the larva must surpass before it can enter metamorphosis on a normal schedule, and the inhibitory action of juvenile hormone on this checkpoint. We examined the effects of nutrition on allatectomized (CAX) larvae that lack juvenile hormone to impose the critical weight checkpoint. Normal larvae respond to prolonged starvation at the start of the last larval stage, by extending their subsequent feeding period to ensure that they begin metamorphosis above critical weight. CAX larvae, by contrast, show no homeostatic adjustment to starvation but start metamorphosis 4 d after feeding onset, regardless of larval size or the state of development of their imaginal discs. By feeding starved CAX larvae for various durations, we found that feeding for only 12-24 h was sufficient to result in metamorphosis on day 4, regardless of further feeding or body size. Manipulation of diet composition showed that protein was the critical macronutrient to initiate this timing. This constant period between the start of feeding and the onset of metamorphosis suggests that larvae possess a molt timer that establishes a minimal time to metamorphosis. Ligation experiments indicate that a portion of the timing may occur in the prothoracic glands. This positive system that promotes molting and the negative control via the critical weight checkpoint provide antagonistic pathways that evolution can modify to adapt growth to the ecological needs of different insects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.