ArticleJournal of visualized experiments : JoVE2013
Isolation of human atrial myocytes for simultaneous measurements of Ca2+ transients and membrane currents.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Modulated Calcium Homeostasis and Release Events Under Atrial Fibrillation and Its Risk Factors: A Meta-Analysis.Frontiers in cardiovascular medicine · 2021Pooled it
- Cytosolic calcium handling signature: integration with clinical predictors enhances prediction of post-operative atrial fibrillation.European heart journal · 2026Article
- Impaired Intracellular Calcium Buffering Contributes to the Arrhythmogenic Substrate in Atrial Myocytes From Patients With Atrial Fibrillation.Circulation · 2024Article
- The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes.Basic research in cardiology · 2023Article
- A modern automated patch-clamp approach for high throughput electrophysiology recordings in native cardiomyocytes.Communications biology · 2022Article
- Neurogranin regulates calcium-dependent cardiac hypertrophy.Experimental and molecular pathology · 2022Article
- Functional isolation, culture and cryopreservation of adult human primary cardiomyocytes.Signal transduction and targeted therapy · 2022Article
- Enhanced Cardiac CaMKII Oxidation and CaMKII-Dependent SR Ca Leak in Patients with Sleep-Disordered Breathing.Antioxidants (Basel, Switzerland) · 2022Article
- Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes.Journal of molecular and cellular cardiology · 2022Article
- Altered atrial cytosolic calcium handling contributes to the development of postoperative atrial fibrillation.Cardiovascular research · 2021Article
- Atrial remodeling and metabolic dysfunction in idiopathic isolated fibrotic atrial cardiomyopathy.International journal of cardiology · 2018Article
- Axial Tubule Junctions Activate Atrial CaFrontiers in physiology · 2018Article
- Axial tubule junctions control rapid calcium signaling in atria.The Journal of clinical investigation · 2016Article
- Oxidized Low-density Lipoprotein (ox-LDL) Cholesterol Induces the Expression of miRNA-223 and L-type Calcium Channel Protein in Atrial Fibrillation.Scientific reports · 2016Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Analysis of tubular membrane networks in cardiac myocytes from atria and ventricles.Journal of visualized experiments : JoVE · 2014Article
- Cellular and molecular mechanisms of atrial arrhythmogenesis in patients with paroxysmal atrial fibrillation.Circulation · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of electrophysiological properties of cardiac ion channels with the patch-clamp technique and the exploration of cardiac cellular Ca(2+) handling abnormalities requires isolated cardiomyocytes. In addition, the possibility to investigate myocytes from patients using these techniques is an invaluable requirement to elucidate the molecular basis of cardiac diseases such as atrial fibrillation (AF).(1) Here we describe a method for isolation of human atrial myocytes which are suitable for both patch-clamp studies and simultaneous measurements of intracellular Ca(2+) concentrations. First, right atrial appendages obtained from patients undergoing open heart surgery are chopped into small tissue chunks ("chunk method") and washed in Ca(2+)-free solution. Then the tissue chunks are digested in collagenase and protease containing solutions with 20 μM Ca(2+). Thereafter, the isolated myocytes are harvested by filtration and centrifugation of the tissue suspension. Finally, the Ca(2+) concentration in the cell storage solution is adjusted stepwise to 0.2 mM. We briefly discuss the meaning of Ca(2+) and Ca(2+) buffering during the isolation process and also provide representative recordings of action potentials and membrane currents, both together with simultaneous Ca(2+) transient measurements, performed in these isolated myocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.