ArticleJournal of visualized experiments : JoVE2013
Improved preparation and preservation of hippocampal mouse slices for a very stable and reproducible recording of long-term potentiation.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Intact corticostriatal function in aged system xTranslational psychiatry · 2025Article
- THz Waves Improve Spatial Working Memory by Increasing the Activity of Glutamatergic Neurons in Mice.Cells · 2025Article
- Dissection of Mouse Hippocampus with Its Dorsal, Intermediate and Ventral Subdivisions Combined with Molecular Validation.Brain sciences · 2022Article
- Sex-regulated gene dosage effect of PPARα on synaptic plasticity.Life science alliance · 2019Article
- Long Term Potentiation in Mouse Hippocampal Slices in an Undergraduate Laboratory Course.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2019Article
- Acupuncture Improves Comorbid Cognitive Impairments Induced by Neuropathic Pain in Mice.Frontiers in neuroscience · 2019Article
- Planar coil-based contact-mode magnetic stimulation: synaptic responses in hippocampal slices and thermal considerations.Scientific reports · 2018Article
- Hippocampal slice preparation in rats acutely suppresses immunoreactivity of microtubule-associated protein (Map2) and glycogen levels without affecting numbers of glia or levels of the glutamate transporter VGlut1.Brain and behavior · 2017Article
- A new method allowing long-term potentiation recordings in hippocampal organotypic slices.Brain and behavior · 2017Article
- Significance of Low Nanomolar Concentration of ZnMolecular neurobiology · 2017Review
- Investigation of hippocampal synaptic transmission and plasticity in mice deficient in the actin-binding protein Drebrin.Scientific reports · 2017Article
- Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents.Journal of visualized experiments : JoVE · 2015Article
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2 authors.
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Abstract
Long-term potentiation (LTP) is a type of synaptic plasticity characterized by an increase in synaptic strength and believed to be involved in memory encoding. LTP elicited in the CA1 region of acute hippocampal slices has been extensively studied. However the molecular mechanisms underlying the maintenance phase of this phenomenon are still poorly understood. This could be partly due to the various experimental conditions used by different laboratories. Indeed, the maintenance phase of LTP is strongly dependent on external parameters like oxygenation, temperature and humidity. It is also dependent on internal parameters like orientation of the slicing plane and slice viability after dissection. The optimization of all these parameters enables the induction of a very reproducible and very stable long-term potentiation. This methodology offers the possibility to further explore the molecular mechanisms involved in the stable increase in synaptic strength in hippocampal slices. It also highlights the importance of experimental conditions in in vitro investigation of neurophysiological phenomena.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.