ArticleNature nanotechnology2013
Molecular crowding shapes gene expression in synthetic cellular nanosystems.
Article in Nature nanotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
94 citing papers in PubMed.
- Engineering macromolecular crowding-driven amyloid-mineral hybrid scaffolds for enhanced bone regeneration.Bioactive materials · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Macromolecule-Driven Supramolecular Polymerization Induced by Crowding Effects.Angewandte Chemie (International ed. in English) · 2025Article
- Designer artificial environments for membrane protein synthesis.Nature communications · 2025Article
- Aging-dependent evolving electrochemical potentials of biomolecular condensates regulate their physicochemical activities.Nature chemistry · 2025Article
- Chromatin conformation, gene transcription, and nucleosome remodeling as an emergent system.Science advances · 2025Article
- Cell-Free Gene Expression in Bioprinted Fluidic Networks.ACS synthetic biology · 2024Article
- Thermo-responsive aqueous two-phase system for two-level compartmentalization.Nature communications · 2024Article
- Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment.Chemical reviews · 2024Review
- Viscosity-dependent control of protein synthesis and degradation.Nature communications · 2024Article
- Macromolecular crowding: Sensing without a sensor.Current opinion in cell biology · 2023Review
- Bayesian inference of polymerase dynamics over the exclusion process.Royal Society open science · 2023Article
- Article
- Protein homeostasis from diffusion-dependent control of protein synthesis and degradation.bioRxiv : the preprint server for biology · 2023Article
- Engineering Cyborg Bacteria Through Intracellular Hydrogelation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Robust and tunable performance of a cell-free biosensor encapsulated in lipid vesicles.Science advances · 2023Article
- Activation/Inhibition of Gene Expression Caused by Alcohols: Relationship with the Viscoelastic Property of a DNA Molecule.Polymers · 2022Article
- Uricase Crowding via Polyelectrolyte Layers Coacervation for Carbon Fiber-Based Electrochemical Detection of Uric Acid.Polymers · 2022Article
- Crowding and confinement act in concert to slow DNA diffusion within cell-sized droplets.iScience · 2022Article
34 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
The integration of synthetic and cell-free biology has made tremendous strides towards creating artificial cellular nanosystems using concepts from solution-based chemistry, where only the concentrations of reacting species modulate gene expression rates. However, it is known that macromolecular crowding, a key feature in natural cells, can dramatically influence biochemical kinetics via volume exclusion effects, which reduce diffusion rates and enhance binding rates of macromolecules. Here, we demonstrate that macromolecular crowding can increase the robustness of gene expression by integrating synthetic cellular components of biological circuits and artificial cellular nanosystems. Furthermore, we reveal how ubiquitous cellular modules, including genetic components, a negative feedback loop and the size of the crowding molecules can fine-tune gene circuit response to molecular crowding. By bridging a key gap between artificial and living cells, our work has implications for efficient and robust control of both synthetic and natural cellular circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.