Evidence map›Paper›PMID 23851345›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014

Regenerating cartilages by engineered ASCs: prolonged TGF-β3/BMP-6 expression improved articular cartilage formation and restored zonal structure.

Chia-Hsin Lu, Tsung-Szu Yeh, Chia-Lin Yeh, Yu-Hua Dean Fang, Li-Yu Sung, Shih-Yeh Lin, Tzu-Chen Yen, Yu-Han Chang, Yu-Chen Hu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.

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  14. 3D Bioprinting of Human Tissues: Biofabrication, Bioinks, and Bioreactors.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Chia-Hsin LuDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Tsung-Szu Yeh1] Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan [2] Division of Cardiovascular Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan.
Chia-Lin YehDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Yu-Hua Dean FangMolecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Li-Yu SungDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Shih-Yeh LinDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
Tzu-Chen Yen1] Department of Nuclear Medicine and Molecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan [2] College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Yu-Han Chang1] College of Medicine, Chang Gung University, Taoyuan, Taiwan [2] Department of Orthopaedic, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Yu-Chen HuDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
National Tsing Hua University · TWChang Gung Memorial Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ASCs) hold promise for cartilage regeneration but their chondrogenesis potential is inferior. Here, we used a baculovirus (BV) system that exploited FLPo/Frt-mediated transgene recombination and episomal minicircle formation to genetically engineer rabbit ASCs (rASCs). The BV system conferred prolonged and robust TGF-β3/BMP-6 expression in rASCs cultured in porous scaffolds, which critically augmented rASCs chondrogenesis and suppressed osteogenesis/hypertrophy, leading to the formation of cartilaginous constructs with improved maturity and mechanical properties in 2-week culture. Twelve weeks after implantation into full-thickness articular cartilage defects in rabbits, these engineered constructs regenerated neocartilages that resembled native hyaline cartilages in cell morphology, matrix composition and mechanical properties. The neocartilages also displayed cartilage-specific zonal structures without signs of hypertrophy and degeneration, and eventually integrated with host cartilages. In contrast, rASCs that transiently expressed TGF-β3/BMP-6 underwent osteogenesis/hypertrophy and resulted in the formation of inferior cartilaginous constructs, which after implantation regenerated fibrocartilages. These data underscored the crucial role of TGF-β3/BMP-6 expression level and duration in rASCs in the cell differentiation, constructs properties and in vivo repair. The BV-engineered rASCs that persistently express TGF-β3/BMP-6 improved the chondrogenesis, in vitro cartilaginous constructs production and in vivo hyaline cartilage regeneration, thus representing a remarkable advance in cartilage engineering.

Indexed as

ChondrogenesisGuided Tissue RegenerationAdipose TissueAnimalsBiomechanical PhenomenaBone Morphogenetic Protein 6CartilageGene ExpressionGene OrderGenetic VectorsMaleRabbitsStem CellsTissue EngineeringTissue ScaffoldsTransduction, GeneticBone Morphogenetic Protein 6Transforming Growth Factor beta3

Identifiers

PMID23851345
PMCPMC3978803
OpenAlexW2150058708

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.